Deoxyhypusine synthase/DHS Protein, Human (His)

The deoxyhorseuridine synthase (DHS) protein plays a crucial role in the NAD-dependent oxidative cleavage of spermidine, followed by the transfer of the butylamine moiety of spermidine to the ε-amino group of the eIF-5A precursor protein. On the key lysine. Deoxyhypusine synthase/DHS Protein, Human (His) is the recombinant human-derived Deoxyhypusine synthase/DHS protein, expressed by E. coli , with C-6*His labeled tag.

Deoxyhypusine synthase (DHS) plays a crucial role in the post-translational modification of the eIF-5A precursor protein. Catalyzing the NAD-dependent oxidative cleavage of spermidine, DHS subsequently transfers the butylamine moiety of spermidine to the epsilon-amino group of a lysine residue on the eIF-5A precursor. This process leads to the formation of the intermediate deoxyhypusine residue, representing the initial step in the conversion of the lysine into an atypical amino acid called hypusine. Hypusination is a unique modification specific to mature eIF-5A and is essential for its proper functioning.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

P49366 (M1-D369)

1725  [NCBI]

Full Length

MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

Approximately 44.52 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 20% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.