DENV2 Envelope Protein (HEK293, His)

NS1 Protein, essential in virus budding, binds the cell membrane, forming a nucleocapsid that becomes the virus core. During entry, NS1 facilitates viral genome penetration post-hemifusion. NS1 migrates to the nucleus, influencing host functions, and counteracts host EXOC1's antiviral effects by sequestering and degrading EXOC1 via the proteasome pathway. Additionally, NS1 impedes RNA silencing by interfering with the host Dicer enzyme. DENV2 Envelope Protein (HEK293, His) is the recombinant DENV2 Envelope protein, expressed by HEK293, with N-His labeled tag.

The NS1 protein plays a vital role in virus budding, as it binds to the cell membrane and assembles the viral RNA into a nucleocapsid, which becomes the core of a fully developed virus particle. In the process of virus entry, NS1 may facilitate the penetration of the viral genome into the host cytoplasm following hemifusion triggered by surface proteins. NS1 has the ability to migrate to the cell nucleus, where it exerts its influence on host functions. It can counteract the antiviral effects of host EXOC1 by sequestering and degrading EXOC1 through the proteasome degradation pathway. Furthermore, NS1 hinders RNA silencing by interfering with the host Dicer enzyme.

Virus

HEK293

N-His

P29990 (M281-S677)

/

Partial

Approximately 55-60 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.