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  3. COL1A1 Protein, Human (HEK293, His)

The procollagen alpha 1/COL1A1 protein is an important type I collagen component and forms a trimeric structure with alpha 2(I) and two alpha 1(I) chains. It interacts with MRC2, associates with MFAP4 in a Ca2+-dependent manner, and interacts with TRAM2. COL1A1 Protein, Human (HEK293, His) is the recombinant human-derived COL1A1 protein, expressed by HEK293, with N-His labeled tag.

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Description

The procollagen alpha 1/COL1A1 protein is an important type I collagen component and forms a trimeric structure with alpha 2(I) and two alpha 1(I) chains. It interacts with MRC2, associates with MFAP4 in a Ca2+-dependent manner, and interacts with TRAM2. COL1A1 Protein, Human (HEK293, His) is the recombinant human-derived COL1A1 protein, expressed by HEK293, with N-His labeled tag.

Background

Pro-Collagen I alpha 1/COL1A1 Protein, a crucial component of type I collagen, is a member of the fibrillar-forming collagen group I. It forms trimeric structures consisting of one alpha 2(I) and two alpha 1(I) chains. The protein interacts with MRC2 and, in a calcium (Ca2+)-dependent manner, associates with MFAP4. Additionally, Pro-Collagen I alpha 1/COL1A1 Protein interacts with TRAM2, as reported in scientific literature. These interactions underline the protein's role in the assembly and structural integrity of type I collagen, contributing to its fundamental function in providing strength and support to various tissues in the body.

Species

Human

Source

HEK293

Tag

N-His

Accession

P02452 (L1229-L1464)

Gene ID

1277

Molecular Construction
N-term
His
COL1A1 (L1229-L1464)
Accession # P02452
C-term
Protein Length

Partial

Synonyms
COL1A1; LCollagen alpha-1(I) chain; LAlpha-1 type I collagen
Predicted Molecular Mass
28.9 kDa
Molecular Weight

Approximately 30-33 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Glycosylation
Yes
Structure/Form
Monomer
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

COL1A1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
COL1A1 Protein, Human (HEK293, His)
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HY-P704237
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