COL1A1 Protein, Human (HEK293, His)

The procollagen alpha 1/COL1A1 protein is an important type I collagen component and forms a trimeric structure with alpha 2(I) and two alpha 1(I) chains. It interacts with MRC2, associates with MFAP4 in a Ca2+-dependent manner, and interacts with TRAM2. COL1A1 Protein, Human (HEK293, His) is the recombinant human-derived COL1A1 protein, expressed by HEK293, with N-His labeled tag.

Pro-Collagen I alpha 1/COL1A1 Protein, a crucial component of type I collagen, is a member of the fibrillar-forming collagen group I. It forms trimeric structures consisting of one alpha 2(I) and two alpha 1(I) chains. The protein interacts with MRC2 and, in a calcium (Ca2+)-dependent manner, associates with MFAP4. Additionally, Pro-Collagen I alpha 1/COL1A1 Protein interacts with TRAM2, as reported in scientific literature. These interactions underline the protein's role in the assembly and structural integrity of type I collagen, contributing to its fundamental function in providing strength and support to various tissues in the body.

Human

HEK293

N-His

P02452 (L1229-L1464)

1277

Partial

Approximately 30-33 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.