CDKN1A Protein, Mouse (His)

CDKN1A Protein, Mouse (His) is the recombinant mouse-derived CDKN1A protein, expressed by E. coli, with C-6*His tag.

The CDKN1A protein assumes a crucial role in controlling cell cycle progression and orchestrating DNA damage-induced G2 arrest. It actively participates in p53/TP53-mediated inhibition of cellular proliferation in response to DNA damage and is also implicated in p53-independent DNA damage-induced G2 arrest, mediated by CREB3L1 in astrocytes and osteoblasts. Functionally, CDKN1A binds to and inhibits cyclin-dependent kinase activity, preventing the phosphorylation of critical cyclin-dependent kinase substrates and thereby blocking cell cycle progression. It contributes to the nuclear localization and assembly of the cyclin D-CDK4 complex, promoting its kinase activity towards RB1. At higher stoichiometric ratios, CDKN1A inhibits the kinase activity of the cyclin D-CDK4 complex and competes with POLD3 for PCNA binding, leading to the inhibition of DNA synthesis by DNA polymerase delta. The intricate regulatory network of CDKN1A includes interactions with HDAC1, MKRN1, PSMA3, CDK4, CDK2, PIM1, STK11, NUAK1, and TRIM39 isoforms, reflecting its diverse role in cell cycle regulation and DNA damage response.

Mouse

E. coli

C-6*His

P39689 (S2-P159)

12575

Full Length of Mature Protein

SNPGDVRPVPHRSKVCRCLFGPVDSEQLRRDCDALMAGCLQEARERWNFDFVTETPLEGNFVWERVRSLGLPKVYLSPGSRSRDDLGGDKRPSTSSALLQGPAPEDHVALSLSCTLVSERPEDSPGGPGTSQGRKRRQTSLTDFYHSKRRLVFCKRKP

Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.