2. Recombinant Proteins
  3. Cytokines and Growth Factors Immune Checkpoint Proteins CD Antigens
  4. TNF Superfamily Stimulatory Immune Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  5. TNF Superfamily Ligands
  6. CD40 Ligand/CD154
  7. CD40L/CD154/TRAP Trimer Protein, Mouse (HEK293, His-Flag)

CD40L/CD154/TRAP Trimer Protein, Mouse (HEK293, His-Flag)

Cat. No.: HY-P705466
Handling Instructions Technical Support

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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Top Publications Citing Use of Products

  • Technical Parameters

  • Properties

  • Documentation

Species

Mouse
Source

HEK293
Tag

N-His;N-Flag
Accession

P27548 (G115-L260)
Gene ID

21947
Molecular Construction
N-term
His-Flag
CD40L (G115-L260)
Accession # P27548
C-term
Protein Length

Full Length of CD40L soluble form
Synonyms
CD40 ligand; CD40-L; TRAP; CD154; sCD40L; TNFSF5
Predicted Molecular Mass
50.5 kDa.
Molecular Weight

Approximately 45-55 kDa, based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
Glycosylation
Yes
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CD40L/CD154/TRAP Trimer Protein, Mouse (HEK293, His-Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD40L/CD154/TRAP Trimer Protein, Mouse (HEK293, His-Flag)
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