2. Metabolic Enzyme/Protease NF-κB Neuronal Signaling Membrane Transporter/Ion Channel TGF-beta/Smad Epigenetics Immunology/Inflammation Apoptosis Cell Cycle/DNA Damage
  3. Angiotensin-converting Enzyme (ACE) IKK Calcium Channel PKC Reactive Oxygen Species (ROS) NF-κB Apoptosis Sirtuin NOD-like Receptor (NLR)
  4. Plantainoside D

Plantainoside D, a phenylethanoid glycosides, is a IKK-β inhibitor with diverse biological activities. Plantainoside D shows inhibitory activity of angiotensin-converting enzyme (ACE) with an IC50 of 2.17 mM. Plantainoside D significantly reduces the release of glutamate from nerve terminals in the cerebral cortex of rats by inhibiting the voltage-dependent calcium channel (VDCCs) and protein kinase C (PKC) signaling cascade. Plantainoside D significantly alleviates cell apoptosis by inhibiting the generation of ROS and the activation of NF-κB. Plantainoside D significantly improves acute lung injury (ALI) induced by sepsis by regulating the Sirt3/NLRP3 signaling pathway. Plantainoside D can be used for the study of neuroprotection, antioxidant, anti-inflammation, antihypertension.

For research use only. We do not sell to patients.

Plantainoside D

Plantainoside D Chemical Structure

CAS No. : 147331-98-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Plantainoside D Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Plantainoside D

View All IKK Isoform Specific Products:

View All Isoforms
IKK-α IKK-β IKK IKKε TBK1

View All Calcium Channel Isoform Specific Products:

View All Isoforms
N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

View All PKC Isoform Specific Products:

View All Isoforms
PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All Sirtuin Isoform Specific Products:

View All Isoforms
Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

View All NOD-like Receptor (NLR) Isoform Specific Products:

View All Isoforms
NLRP3 NLRP1 NOD1 NOD2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Plantainoside D, a phenylethanoid glycosides, is a IKK-β inhibitor with diverse biological activities. Plantainoside D shows inhibitory activity of angiotensin-converting enzyme (ACE) with an IC50 of 2.17 mM. Plantainoside D significantly reduces the release of glutamate from nerve terminals in the cerebral cortex of rats by inhibiting the voltage-dependent calcium channel (VDCCs) and protein kinase C (PKC) signaling cascade. Plantainoside D significantly alleviates cell apoptosis by inhibiting the generation of ROS and the activation of NF-κB. Plantainoside D significantly improves acute lung injury (ALI) induced by sepsis by regulating the Sirt3/NLRP3 signaling pathway. Plantainoside D can be used for the study of neuroprotection, antioxidant, anti-inflammation, antihypertension[1][2][3][4][5][6].

IC50 & Target[1][2][5][6]

IKK-β

 

Calcium Channel

 

NF-κB

 

NLRP3

 

ACE

2.17 mM (IC50)

PKC

 

In Vitro

Plantainoside D (0-50 μM, 10 min) significantly inhibits the release of glutamate induced by 4-aminopyridine (4-AP) (HY-B0604) via vesicle exocytosis pathway with an IC50 of 32 μM in rat synaptosome[1].
Plantainoside D (30 μM, 10 min) reduces the Ca²⁺ influx induced by 4-AP through N-type calcium channels, and does not alter the membrane potential in rat synaptosome[1].
Plantainoside D (30 μM, 20 min) acts through PKC-α/SNAP-25 phosphorylation as its key downstream mechanism in rat synaptosome[1].
Plantainoside D (5 μM, 24 h) significantly improves sepsis induced ALI by regulation of Sirt3/NLRP3 pathway in MLE-12 cells[2].
Plantainoside D (1-20 μg/mL, 1-4 h) significantly reduces the apoptosis induced by Doxorubicin (ADR) (HY-15142A) by inhibiting the generation of ROS and the activation of NF-κB[3].
Plantainoside D exhibits weak inhibitory effects of CYP1A2 (IC50 = 12.83 μM), CYP2D6 (IC50 = 8.39 μM) and CYP3A4 (IC50 = 14.66 μM)[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Plantainoside D Related Antibodies

Western Blot Analysis[1]

Cell Line: Rat cerebral cortical synaptosomes
Concentration: 30 μM
Incubation Time: 20 min
Result: Reduced the phosphorylation of PKC-α (from 239.2% to 54.1%) and SNAP-25 (from 228.9% to 89.7%) induced by 4-AP.

Cell Viability Assay[2]

Cell Line: MLE-12 cells induced by LPS (HY-D1056)
Concentration: 5 μM
Incubation Time: 24 h
Result: Increased cell vitality, reduced ROS and MDA.

Apoptosis Analysis[3]

Cell Line: H9c2 cell with ADR
Concentration: 1, 5, 10, 20 μg/mL
Incubation Time: 1 h
Result: Reduced the cell mortality rate with 20 μg/mL pretreatment.
Reduced apoptotic morphology (nuclear enrichment, fragmentation).
Increased Bcl-2/Bax ratio and inhibited caspase-3.

Western Blot Analysis[3]

Cell Line: H9c2 cell with ADR
Concentration: 2 μM
Incubation Time: 15 min, 30 min, 1h, 2h, 4h
Result: Abrogated the activation of NF-κB.
The glycoside reduced the degradation of IkBα in ADR-treated cells.
In Vivo

Plantainoside D (50 mg/kg, i.p., once daily for 7 days) significantly improves sepsis induced ALI by regulation of Sirt3/NLRP3 pathway in mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: LPS-induced ALI model established in ICR WT male mice (6 weeks, 16-18 g)[2]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection (i.p.), once daily for 7 days
Result: Significantly decreased the levels of w/d, ROS and MPO.
Significantly decreased MDA and increased SOD and GSH-px in serum and lung tissues.
Significantly restored oxidative stress and neutrophil infiltration.
Significantly decreased the levels of cytokines TNF-α, IL-1β, IL-6, NLRP3, ASC and caspase-1.
Molecular Weight

640.59

Formula

C29H36O16
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O[C@H]([C@H]([C@@H]([C@@H](COC(/C=C/C1=CC=C(O)C(O)=C1)=O)O2)O)O[C@H]3[C@@H]([C@H]([C@@H]([C@@H](CO)O3)O)O)O)[C@@H]2OCCC4=CC=C(O)C(O)=C4
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (390.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5611 mL 7.8053 mL 15.6106 mL
5 mM 0.3122 mL 1.5611 mL 3.1221 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (3.25 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.92%

SDS (252 KB)

COA (250 KB) HNMR (347 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5611 mL 7.8053 mL 15.6106 mL 39.0265 mL
5 mM 0.3122 mL 1.5611 mL 3.1221 mL 7.8053 mL
10 mM 0.1561 mL 0.7805 mL 1.5611 mL 3.9027 mL
15 mM 0.1041 mL 0.5204 mL 1.0407 mL 2.6018 mL
20 mM 0.0781 mL 0.3903 mL 0.7805 mL 1.9513 mL
25 mM 0.0624 mL 0.3122 mL 0.6244 mL 1.5611 mL
30 mM 0.0520 mL 0.2602 mL 0.5204 mL 1.3009 mL
40 mM 0.0390 mL 0.1951 mL 0.3903 mL 0.9757 mL
50 mM 0.0312 mL 0.1561 mL 0.3122 mL 0.7805 mL
60 mM 0.0260 mL 0.1301 mL 0.2602 mL 0.6504 mL
80 mM 0.0195 mL 0.0976 mL 0.1951 mL 0.4878 mL
100 mM 0.0156 mL 0.0781 mL 0.1561 mL 0.3903 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Plantainoside D Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Plantainoside D147331-98-4Angiotensin-converting Enzyme (ACE)IKKCalcium ChannelPKCReactive Oxygen Species (ROS)NF-κBApoptosisSirtuinNOD-like Receptor (NLR)IκB kinaseI kappa B kinase Ca2+ channelsCa channelsProtein kinase CNuclear factor-κBNuclear factor-kappaBGlutamate releaseVoltage-dependent Ca2+ channelCerebral cortexSynaptosomesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Plantainoside D
Cat. No.:
HY-N5063
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.