1. Neuronal Signaling
  2. Beta-secretase Amyloid-β
  3. NB-360

NB-360 is a potent, brain penetrable and orally active β‐secretase 1/2 (BACE1/BACE2) dual inhibitor with IC50 values of 5 and 6 nM. NB-360 can inhibit amyloid-β protein accumulation. NB-360 can be used for the researches of inflammation and neurological disease, such as Alzheimer's disease.

For research use only. We do not sell to patients.

NB-360

NB-360 Chemical Structure

CAS No. : 1262857-73-7

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Description

NB-360 is a potent, brain penetrable and orally active β‐secretase 1/2 (BACE1/BACE2) dual inhibitor with IC50 values of 5 and 6 nM. NB-360 can inhibit amyloid-β protein accumulation. NB-360 can be used for the researches of inflammation and neurological disease, such as Alzheimer's disease[1][2].

IC50 & Target[1]

BACE1

5 nM (IC50)

BACE2

6 nM (IC50)

In Vitro

NB-360 inhibits Aβ40 release in APP-overexpressing CHO cells, with an IC50 of 3 nM in wtAPP CHO cells and 33 nM in SweAPP CHO cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NB-360 (100 μmol/kg, i.g., daily for 6 weeks) blocks the progression of Aβ deposition and inflammation in APP transgenic mice[1].
NB-360 (1.5-14.5 mg/kg, p.o.) reduces Aβ production in rats[2].
NB-360 (45 mg/kg, p.o., daily for 6 weeks) reduces Aβ40 levels in C57/BL6 mice[2].
NB-360 (30 μmol/kg, p.o., a single dose) reduces Aβ40 levels in young APP51/16 mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: APP transgenic mice models[1]
Dosage: 100 μmol/kg
Administration: Orally gavage, daily for 6 weeks
Result: Reduced Aβ levels in brain.
Stopped accumulation of activated inflammatory cells in brain.
Molecular Weight

449.40

Formula

C21H19F4N5O2

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NC=C(C#N)C=C1C)NC2=CC=C(F)C([C@]3(C)CO[C@](C(F)(F)F)(C)C(N)=N3)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (556.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2252 mL 11.1259 mL 22.2519 mL
5 mM 0.4450 mL 2.2252 mL 4.4504 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2252 mL 11.1259 mL 22.2519 mL 55.6297 mL
5 mM 0.4450 mL 2.2252 mL 4.4504 mL 11.1259 mL
10 mM 0.2225 mL 1.1126 mL 2.2252 mL 5.5630 mL
15 mM 0.1483 mL 0.7417 mL 1.4835 mL 3.7086 mL
20 mM 0.1113 mL 0.5563 mL 1.1126 mL 2.7815 mL
25 mM 0.0890 mL 0.4450 mL 0.8901 mL 2.2252 mL
30 mM 0.0742 mL 0.3709 mL 0.7417 mL 1.8543 mL
40 mM 0.0556 mL 0.2781 mL 0.5563 mL 1.3907 mL
50 mM 0.0445 mL 0.2225 mL 0.4450 mL 1.1126 mL
60 mM 0.0371 mL 0.1854 mL 0.3709 mL 0.9272 mL
80 mM 0.0278 mL 0.1391 mL 0.2781 mL 0.6954 mL
100 mM 0.0223 mL 0.1113 mL 0.2225 mL 0.5563 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
NB-360
Cat. No.:
HY-124322
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