Licoisoflavone A

Name: Licoisoflavone A
Brand: MedChemExpress
SKU: HY-N3389
Rating: 4.5 (1 reviews)

Cat. No.: HY-N3389 Purity: 99.41%: Data Sheet
COA

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Licoisoflavone A is an orally active isoflavone. Licoisoflavone A inhibits proliferation, induces apoptosis, and causes G1/S phase arrest in colorectal cancer (CRC) cells. Licoisoflavone A inhibits the CDK2-Cyclin E1 axis. Licoisoflavone A inhibits lipid peroxidation with an IC₅₀ of 7.2 μM. Licoisoflavone A shows a dose-dependent inhibition effect on SARS-CoV-2 infection. Licoisoflavone A exhibits significant anti-tumor efficacy in mice bearing CT26 cell subcutaneous xenografts. Licoisoflavone A can be used for the study of colorectal cancer and SARS-CoV-2 infection.

For research use only. We do not sell to patients.

Licoisoflavone A Chemical Structure

CAS No. : 66056-19-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Licoisoflavone A:

(S)-Licoisoflavone A Get quote

1 Publications Citing Use of MCE Licoisoflavone A

•Food Chem. 2025 May 31:489:144992. [Abstract]

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Sirtuin Isoform Specific Products:

View All Isoforms

Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

View All CDK Isoform Specific Products:

View All Isoforms

CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Licoisoflavone A (0-100 μM) inhibits RNA-dependent RNA polymerase (RdRp) and cAMP-specific 3’,5’-cyclic phosphodiesterase 4 (PDE4) with IC₅₀ values of 47.31 μM and 1.77 μM, respectively^[1].
Licoisoflavone A (5 μM, 2 days) significantly reduces the cross-sectional area of Ang II-induced hypertrophic H9c2 cells by approximately 46%^[2].
Licoisoflavone A (15 μM, 48 h) upregulates Sirt3 expression and reduces MnSOD acetylation at K68 in PE-induced neonatal rat cardiomyocytes^[2].
Licoisoflavone A (15 μM, 48 h) decreases relative ANF and BNP levels in Phenylephrine (PE) (HY-B0769)-induced hypertrophic cardiac cells, but this effect is blocked when co-treated with Sirt3 inhibitor 3-TYP (HY-108331)^[2].
Licoisoflavone A ( 6.25-25 μM, 48 h) significantly inhibits proliferation, induces apoptosis, and causes G1/S phase arrest in HCT116 and SW480 colorectal cancer (CRC) cells^[4].
Licoisoflavone A (6.25-25 μM, 48 h) downregulates CDK2, Cyclin E1, and phosphorylated Rb (p-Rb) while upregulating p27 in HCT116 and SW480 cells, inhibiting the CDK2-Cyclin E1 axis^[4].
Licoisoflavone A (1-1000000 nM, 3 days) dose-dependently inhibits viability of 5 patient-derived CRC organoids (CRCOs) with IC₅₀ values ranging from 7.02 to 82.98 μM, and shows lower toxicity to normal colorectal organoids (CNOs)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[2]

Cell Line:	PE-induced hypertrophic cardiac cells
Concentration:	15 μM
Incubation Time:	48 h
Result:	Decreased relative ANF and BNP levels in PE-induced hypertrophic cardiac cells, but this effect is blocked when co-treated with Sirt3 inhibitor 3-TYP.

Cell Cycle Analysis^[4]

Cell Line:	HCT116 and SW480 colorectal cancer (CRC) cells
Concentration:	6.25, 12.5, 25 μM
Incubation Time:	48 h
Result:	CausesdG1/S phase arrest in HCT116 and SW480 colorectal cancer (CRC) cells.

Apoptosis Analysis^[4]

Cell Line:	HCT116 and SW480 colorectal cancer (CRC) cells
Concentration:	6.25, 12.5, 25 μM
Incubation Time:	48 h
Result:	Induced apoptosis in HCT116 and SW480 colorectal cancer (CRC) cells.

Western Blot Analysis^[4]

Cell Line:	HCT116 and SW480 colorectal cancer (CRC) cells
Concentration:	6.25, 12.5, 25 μM
Incubation Time:	48 h
Result:	Downregulated CDK2, Cyclin E1, and phosphorylated Rb (p-Rb). Upregulated p27.

In Vivo

Licoisoflavone A (25-50 mg/kg, p.o., daily, 2 weeks) exhibits significant anti-tumor efficacy in BALB/c mice bearing CT26 cell subcutaneous xenografts ^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CT26.WT cells (10⁶ in 100 μL) were subcutaneously inoculated into the right axillary region of 6-week-old male BALB/c SPF mice^[4].
Dosage:	25, 50 mg/kg
Administration:	p.o. daily for 2 weeks
Result:	Achieved dose-dependent tumor growth inhibition in BALB/c mice bearing CT26 cell xenografts, with reduced tumor weight and volume at 25 mg/kg and 50 mg/kg doses. Showed no significant toxicity, with no abnormal changes in liver and spleen histopathology and serum ALT/AST levels. Reduced the expression of CDK2 and Cyclin E1 in tumor tissues, while increased p27 expression. Inhibited phosphorylation of Rb in tumor tissues.

Molecular Weight

354.35

Formula

C₂₀H₁₈O₆

CAS No.

66056-19-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(C2=C(C(C/C=C(C)/C)=C(O)C=C2)O)=COC3=CC(O)=CC(O)=C13

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (282.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8221 mL	14.1103 mL	28.2207 mL
5 mM	0.5644 mL	2.8221 mL	5.6441 mL
10 mM	0.2822 mL	1.4110 mL	2.8221 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.41%

Select Batch:

Data Sheet (280 KB) SDS (393 KB)

COA (247 KB) HNMR (188 KB) CNMR (191 KB) LCMS (96 KB)

Handling Instructions (2659 KB)

References

[1]. Xu H, et al. Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19. Proc Natl Acad Sci U S A. 2023 May 2;120(18):e2301775120. [Content Brief]

[2]. Guo R, et al. High content screening identifies licoisoflavone A as a bioactive compound of Tongmaiyangxin Pills to restrain cardiomyocyte hypertrophy via activating Sirt3. Phytomedicine. 2020 Mar;68:153171. [Content Brief]

[3]. S. Toda, et al. Inhibitory Effects of Isoflavones in Sophora mooracrotiana on Lipid Peroxidation by Superoxide. Pharmaceutical Biology. 2002, 40 (6):422-424.

[4]. Cui J, et al. Licoisoflavone A inhibits colorectal cancer cell proliferation through targeting CDK2-Cyclin E1 axis-mediated cell cycle transition. Biochem Pharmacol. 2025 Oct;240:117124. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8221 mL	14.1103 mL	28.2207 mL	70.5517 mL
	5 mM	0.5644 mL	2.8221 mL	5.6441 mL	14.1103 mL
	10 mM	0.2822 mL	1.4110 mL	2.8221 mL	7.0552 mL
	15 mM	0.1881 mL	0.9407 mL	1.8814 mL	4.7034 mL
	20 mM	0.1411 mL	0.7055 mL	1.4110 mL	3.5276 mL
	25 mM	0.1129 mL	0.5644 mL	1.1288 mL	2.8221 mL
	30 mM	0.0941 mL	0.4703 mL	0.9407 mL	2.3517 mL
	40 mM	0.0706 mL	0.3528 mL	0.7055 mL	1.7638 mL
	50 mM	0.0564 mL	0.2822 mL	0.5644 mL	1.4110 mL
	60 mM	0.0470 mL	0.2352 mL	0.4703 mL	1.1759 mL
	80 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8819 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7055 mL