2. MCE Kits
  3. Cell Biology
  4. Cell Culture
  5. Cell Transfection
  6. PEI Transfection Reagent

PEI Transfection Reagent 

Cat. No.: HY-K2014
Manual COA SDS Technical Support

MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It has high-efficiency, low-toxicity, strong-stability, and is suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production.

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    MedChemExpress Validation
    Experimental conditions:
    Cell density: 50% of the density during cell transfection
    Dosage: 1 μg of GFP plasmid + 2.5 μL of transfection reagent
    Detection scheme: Detection 48 h after transfection
    The results showed that PEI Transfection Reagent (HY-K2014) showed better transfection performance for GFP plasmid transfection in 293T, CHO, HCT116, HT29, and U2OS cells.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    PEI is a commonly used polymer carrier in gene transfection. It consists of cationic polymers and can introduce nucleic acids into eukaryotic cells. In polymer-based transfection, exogenous DNA forms complexes with cationic polymers that enter host cells by endocytosis.

    MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It is modified by introducing functional genes to enhance the binding ability of DNA, and reduced the cytotoxicity. In addition, the cell uptake and the endosome escape functions of PEI modified was enhanced significantly.

    MCE PEI Transfection Reagent is high-efficiency, low-toxicity, strong-stability, and suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production.

    Storage

    -20°C, 1 year.

    Avoid repetitive freeze-thaw cycles.

    Components
    Components HY-K2014-1 mL HY-K2014-5 mL HY-K2014-10 mL
    PEI Transfection Reagent 1 mL 1 mL × 5 1 mL × 10
    Documentation

    Manual (980 KB) SDS (177 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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