Indocyanine green (solution) 

Indocyanine green (Foxgreen) (solution) is a low toxicic fluorescent agent that has been widely used in medical diagnostics, such as determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography (Ex/Em = 785/813 nm)^[1].
Solution Concentration: 10 mM

Indocyanine green (Foxgreen)-photodynamic therapy (ICG-PDT) at concentrations 1000 μg/mL, induces the significant expression of BAX in HGF cells; however, the laser irradiation as well as ICG shows no significant effects on the expression of BCL-2 gene^[1]. 1. Solution preparation^[3]
1.1 Stock solution
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute stock solution with Tyrode’s solution or other buffers (optimized according to the experiment). The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Tissue staining
2.1 Experiments are performed on Langendorff-perfused hearts from New Zealand White rabbits.
2.2 Blebbistatin (20?μM) (HY-13813) and Biacetyl monoxime (5?mM) (HY-Y0413) are added to the perfusate to suppress contractions while allowing electrical activity to continue.
2.3 The heart is stained by adding increasing concentrations (from 0.03?μM to 10?μM) of the Indocyanine green dye to the perfusate.
2.4 Optical signals recorded with a spectrometer are collected for periods of 3?sec in duration.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Description^[2].
Indocyanine green (ICG) can absorb the near IR laser energy and release heat in the dyed tissue. Pulsed laser treatment combined with Indocyanine green causes targeted tumor cell death in neuroblastoma tumor without capsule.
Indocyanine green injections and application of laser.
After tumor volume reached 1000 mm³, mice are anesthetized underwent laparotomy to expose the orthotopic neuroblastoma tumor, liver, and kidney; skin over the proximal leg is removed to expose the quadriceps.
Exposed tumor and organs are treated at multiple locations with a pulse or continuous wave laser (805 nm), or laser application after an injection of 10 μL of 0.857 mg/mL Indocyanine green (ICG).
Laser exposure is limited to a 3 mm diameter area for 20-120 seconds. Total energy delivered per treatment area is recorded.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

774.97

C₄₃H₄₇N₂NaO₆S₂

3599-32-4

O=S(CCCC[N+]1=C(/C=C/C=C/C=C/C=C2N(CCCCS(=O)(O[Na])=O)C3=C(C4=CC=CC=C4C=C3)C/2(C)C)C(C)(C)C5=C1C=CC6=CC=CC=C56)([O-])=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Gharesi S, et al. Effect of photodynamic therapy based on indocyanine green on expression of apoptosis-related genes in human gingival fibroblast cells. Photodiagnosis Photodyn Ther. 2017 Apr 21.  [Content Brief]

  [2]. Taylor JS, et al. Combined application of Indocyanine green (ICG) and laser lead to targeted tumor cell destruction. J Pediatr Surg. 2018;53(12):2475-2479.  [Content Brief]

  [3]. Mačianskienė R, et al. Spectral characteristics of voltage-sensitive indocyanine green fluorescence in the heart. Sci Rep. 2017;7(1):7983. Published 2017 Aug 11.  [Content Brief]