Filipin complex (solution) 

Filipin complex (solution)is a potent polyene macrolide antifungal antibiotic. Filipin complex inserts into membranes and sequester cholesterol into complexes and inhibits PRRSV entry. The Filipin complex consists of about 75.8% Filipin III (HY-N6718), 10.8% Filipin IV, 9.1% Filipin II, and 1.2% Filipin I (Ex/Em = 380/430 nm)^[1][2][3].
Solution Concentration: 5 mg/mL

1. Solution preparation^[4]
1.1 Preparation of working solution
Dilute to 50 μg/mL with PBS or serum-free cell culture medium (optimized according to the experiment).
Note: The working solution should be prepared and used immediately, and protected from light.
The stock soultion, store at -20°C or -80°C away from light and avoid repeated freezing and thawing.

For neuronal cholesterol levels staining.
1. The staining sections are washed three times with PBS and incubated with 1.5 mg/mL glycine for 10 min at room temperature.
2. Then sections are stained with 0.05 mg/mL Filipin complex in PBS containing 10% FBS for 2 h and rinsed for three times with PBS.
3. Use an Olympus FV10i confocal microscope for image (Ex/Em = 380/430 nm).

Filipin complex (2 μg/mL; 1+48 h) inhibits PRRSV entry with respiratory syndrome virus (PRRSV) genome copies in infected cells reduced by 61% in MARC-145 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

11078-21-0

[Filipin complex (solution)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Malcolm E. Bergy et al. Filipin complex. Biochemistry, 1968, 7, 2, 653-659.

  [2]. Houdai T, et al. Membrane-permeabilizing activities of amphidinol 3, polyene-polyhydroxy antifungal from a marine dinoflagellate. Biochim Biophys Acta. 2004 Nov 17;1667(1):91-100.  [Content Brief]

  [3]. Huang L, et al. Role of lipid rafts in porcine reproductive and respiratory syndrome virus infection in MARC-145 cells. Biochem Biophys Res Commun. 2011 Oct 28;414(3):545-50.  [Content Brief]

  [4]. Huang S, et al. Chimeric cerebral organoids reveal the essentials of neuronal and astrocytic APOE4 for Alzheimer's tau pathology. Signal Transduct Target Ther. 2022 Jun 13;7(1):176.  [Content Brief]