DiBAC4(3) (solution) 

DiBAC4(3) (solution) is a voltage-sensitive fluorescent dye (λ_ex=490 nm, λ_em=505 nm). DiBAC4(3) can be used to measure cell membrane potential.
Solution Concentration: 10 mM

1. Solution preparation
1.1 Stock solution
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute with Hanks and 20 mM Hepes buffer (HHBS) or other buffer, such as pH 7 with 0.04% to 0.08% Poloxamer 407 (F127) (HY-D1005) and 2 mM Trypan Red (HY-D0983)). The corresponding stock solution can be diluted according to the actual situation. Note that if the solvent is DMSO, the cytotoxicity of DMSO must be considered, and a solvent control should be prepared; if the solvent is pure water, the working solution needs to be filtered and sterilized before adding cells.
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Cell staining
2.1 Cells are plated in clear-bottomed black 96-well microplates.
2.2 Add 100 μL DiBAC4(3) into the cell plate.
2.3 Incubate the dye-loading plate in a cell incubator for 30 to 60 minutes.
2.4 Run the membrane potential assay by monitoring the fluorescence intensity at Ex/Em = 490/505 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

516.64

C₂₇H₄₀N₄O₆

70363-83-6

O=C1N(CCCC)C(/C(C(N1CCCC)=O)=C/C=C/C(C(N2CCCC)=O)C(N(CCCC)C2=O)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.  [Content Brief]