Deoxymannojirimycin hydrochloride 

Deoxymannojirimycin hydrochloride is a selective class I α1,2-mannosidase inhibitor with an IC₅₀ of 20 μM. Deoxymannojirimycin hydrochloride is also a N-linked glycosylation inhibitor. Deoxymannojirimycin hydrochloride has antiviral activity against HIV‐1 strains . Deoxymannojirimycin hydrochloride increases high mannose structures. Deoxymannojirimycin hydrochloride can be used for the study of liver cancer and colon cancer^{[1][2][3][4][5][6]}.

Deoxymannojirimycin (2 mM; 24 h) hydrochloride inhibits Golgi α-mannosidase I and leads to an increase in high mannose structures in P815 and EL-4 cells^[1].
Deoxymannojirimycin (4-5 days) hydrochloride shows antiviral efficacy against mutant HIV-1 strains in CEM cell cultures, with EC₅₀ values ranging between 90 and 155 μM^[2].
Deoxymannojirimycin (100-250 μM) hydrochloride potentiates the antiviral efficacy of CBAs (HHA and GNA) against wild-type HIV-1 replication in CEM cell cultures^[2].
Deoxymannojirimycin (1 mM; 8 h) hydrochloride induces the activation and up-regulation of key molecules of ER stress-UPR, such as GRP78/Bip and XBP1, in human hepatocarcinoma 7721 cells^[3].
Deoxymannojirimycin (150 μM; 15 h) hydrochloride causes the accumulation of Man8GlcNAc2 on 97-kDa HMG-CoA reductase in UT-1 cells^[4].
Deoxymannojirimycin (2 mM) hydrochloride completely inhibits α-mannosidase I activity in both differentiated and undifferentiated HT-29 cells^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

163.17

C₆H₁₃NO₄

84444-90-6

OC[C@@H]1[C@H]([C@@H]([C@@H](CN1)O)O)O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Bagriaçik EU, et al. Glycosylation of native MHC class Ia molecules is required for recognition by allogeneic cytotoxic T lymphocytes. Glycobiology. 1996 Jun;6(4):413-21.  [Content Brief]

  [2]. Balzarini J. The alpha(1,2)-mannosidase I inhibitor Deoxymannojirimycin potentiates the antiviral activity of carbohydrate-binding agents against wild-type and mutant HIV-1 strains containing glycan deletions in gp120. FEBS Lett. 2007 May 15;581(10):2060-4.  [Content Brief]

  [3]. Lu Y, et al. Deoxymannojirimycin, the alpha1,2-mannosidase inhibitor, induced cellular endoplasmic reticulum stress in human hepatocarcinoma cell 7721. Biochem Biophys Res Commun. 2006 May 26;344(1):221-5.  [Content Brief]

  [4]. Bischoff J, et al. The use of Deoxymannojirimycin to evaluate the role of various alpha-mannosidases in oligosaccharide processing in intact cells. J Biol Chem. 1986 Apr 5;261(10):4766-74.  [Content Brief]

  [5]. Ogier-Denis E, et al. Dual effect of Deoxymannojirimycin on the mannose uptake and on the N-glycan processing of the human colon cancer cell line HT-29. J Biol Chem. 1990 Apr 5;265(10):5366-9.  [Content Brief]

  [6]. F Vallee, et al. Structural Basis for Catalysis and Inhibition of N-glycan Processing Class I Alpha 1,2-mannosidases. J Biol Chem. 2000 Dec 29;275(52):41287-98.  [Content Brief]