SAM68 Antibody (YA2136)

SAM68 Antibody (YA2136) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2136), targeting SAM68, with a predicted molecular weight of 48 kDa (observed band size: 68 kDa). SAM68 Antibody (YA2136) can be used for WB, IHC-P, ICC/IF experiment in human, mouse, rat background.

SAM68 is an RNA-binding protein recruited and tyrosine-phosphorylated by multiple receptor systems (e.g., T-cell, leptin, and insulin receptors). Upon phosphorylation, it functions as an adapter in signal transduction by binding SH2/SH3 domain-containing proteins. It regulates G2-M cell cycle progression, represses CBP-dependent transcription by competing for CBP binding, and acts as a putative modulator of mRNA stability/translation and nuclear export. It enhances the association of CTE-containing mRNA with polyribosomes for translation initiation, though some studies exclude its role in exporting unspliced CTE-RNA. SAM68 binds RNA with a bipartite 5'-[AU]UAA-3' motif (spaced >15 nucleotides) and poly(A), influencing alternative splicing—e.g., CD44 (Ras-dependent), BCL2L1 (promoting Bcl-X(S) with HNRNPA1), and NRXN1/NRXN3 (via AS4 segment). Synergistic with KHDRBS2/SLIM-1 in activity-dependent NRXN1 exon skipping but antagonistic for NRXN3 (By similarity). Isoform 3, exclusive to growth-arrested cells, inhibits S-phase progression.

Q07666

Predicted band size: 48 kDa; Observed band size: 68 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104500

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using SAM68 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using SAM68 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using SAM68 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human SAM68