RUNX1/2/3 Antibody (YA086)

RUNX2 Antibody (YA086) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RUNX2.

RUNX1/2/3 forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX family members modulate target gene transcription by recognizing the core consensus binding sequence 5'-TGTGGT-3' (or rarely 5'-TGCGGT-3') in regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances RUNX's DNA-binding specificity. The heterodimer binds core sites of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3, and GM-CSF promoters. Essential for normal hematopoiesis, RUNX1 synergizes with ELF4 to transactivate the IL-3 promoter and with ELF2 for the BLK promoter. It inhibits KAT6B-dependent transcriptional activation (by similarity) and regulates lineage commitment of immature T-cell precursors. CBF complexes repress ZBTB7B during cytotoxic (CD8+) T-cell development by binding RUNX sites in the ZBTB7B locus, acting as a transcriptional silencer to enable cytotoxic T-cell differentiation. RUNX1 also controls regulatory T-cell (Treg) anergy and suppressive function via FOXP3 association, activating IL2 and IFNG while downregulating TNFRSF18, IL2RA, and CTLA4 in conventional T-cells (by similarity). In T-helper 17 cells, it positively regulates RORC expression. Isoform AML-1G exhibits higher binding affinity for target genes (e.g., TCR-beta-E2 and RAG-1 sites) but is less effective in neutrophil differentiation. Isoform AML-1L interferes with RUNX1 transactivation activity.

Free

Q01196 / Q13761 / Q13950

Predicted band size: 49 kDa; Observed band size: 49 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102992

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Western blot analysis of extracts from C6 (lane 2(20μg), RAW264.7 (lane 3(20μg) and Jurkat (lane 4(20μg) using RUNX (HY-P80888) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of C2C12 cells labeling RUNX Antibody(HY-P80888) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RUNX Antibody (HY-P80888) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Saos-2 cells labeling RUNX Antibody(HY-P80888) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RUNX Antibody (HY-P80888) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using RUNX Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80888, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using RUNX Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80888, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-F, IHC-P, ICC/IF, IP

Liquid

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Q01196 aa440-453.