Ret Antibody (YA097)

Ret Antibody (YA097) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ret.

The RET protein, a receptor tyrosine-protein kinase, plays a crucial role in diverse cellular processes such as cell proliferation, neuronal navigation, migration, and differentiation upon binding with glial cell-derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates the delicate balance between cell death and survival, as well as positional information. Essential for the molecular coordination during intestine organogenesis, RET is involved in the development of the enteric nervous system, renal organogenesis, and promotes the formation of Peyer's patch-like structures in the gut-associated lymphoid tissue. Furthermore, RET modulates cell adhesion, mediated by caspase cleavage in sympathetic neurons, and facilitates cell migration in an integrin-dependent manner. Operating as a dependence receptor, it triggers apoptosis in the absence of the ligand GDNF in somatotrophs but promotes survival and downregulates growth hormone production in its presence. RET is a key mediator in various diseases, particularly neuroendocrine cancers characterized by aberrant integrins-regulated cell migration. Additionally, it mediates GDF15-induced cell signaling in the brainstem through interaction with GFRAL, resulting in the inhibition of food intake and activation of MAPK- and AKT-signaling pathways. Notably, isoform 1 in complex with GFRAL induces higher activation of the MAPK-signaling pathway compared to isoform 2 in the same complex.

Free

P07949

Predicted band size: 124 kDa;Observed band size: 155,185 kDa

Protein A affinity purified.

Cell membrane, Endosome membrane.

Non-conjugated

Unmodified

AB_3102103

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:2000; ICC/IF: 1:100-1:500; IHC-P: 1:500; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from THP-1(lane 2(20μg) ) and THP-1 (lane 3(20μg) using Ret (HY-P80307) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80307, 1/10, 000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Ret Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80307, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using Ret Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80307, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunocytochemistry analysis of NIH3T3 cells labeling Ret with Ret antibody (HY-P80307) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Ret antibody (HY-P80307) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF, IHC-P, IP, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Ret.AA range:1065-1114.