Phospholipase C gamma 1 Antibody (YA179)

Phospholipase C gamma 1 Antibody (YA179) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospholipase C gamma 1.

Phospholipase C gamma 1 (PLCγ1) mediates the production of second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), playing a crucial role in regulating intracellular signaling cascades. It becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, EGFR, FGFR1, FGFR2, FGFR3, and FGFR4 (By similarity). PLCγ1 is involved in actin reorganization and cell migration. Additionally, it acts as a guanine nucleotide exchange factor, binding to the GTPase DNM1 and catalyzing GDP dissociation to facilitate GTP binding, thereby enhancing DNM1-dependent endocytosis (By similarity).

Free

P19174

Predicted band size: 149 kDa;Observed band size: 149 kDa

Protein A affinity purified.

Cell projection.

Non-conjugated

Unmodified

AB_3102510

Cardiovascular

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000; ICC: 1:50-1:200; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from RAW264.7(lane 2(10μg) or lane 3(20μg) ) and NIH3T3 (lane 4(10μg) or lane 5(20μg) ) using Phospholipase C gamma 1(HY-P80427) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80427, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IP

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Phospholipase C gamma 1.AA range:690-852.