Phospho-Src (Tyr419) Antibody (YA150)

Phospho-Src (Tyr419) Antibody (YA150) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Src (Tyr419).

Src, a non-receptor protein tyrosine kinase, emerges as a central player in an array of cellular processes triggered by the engagement of diverse receptors, including immune response receptors, integrins, receptor protein tyrosine kinases, G protein-coupled receptors, and cytokine receptors. It orchestrates signaling pathways governing gene transcription, immune responses, cell adhesion, cell cycle progression, apoptosis, migration, and cellular transformation. While functional redundancy within the SRC kinase family complicates the identification of specific roles, SRC appears to be a primary kinase activated upon receptor engagement, influencing the activation of other protein tyrosine kinase (PTK) families. SRC is implicated in the regulation of cytoskeletal organization by phosphorylating substrates like AFAP1 and cortactin (CTTN). Its involvement extends to cell-cell and cell-matrix junctions, where it phosphorylates components such as beta-catenin (CTNNB1), delta-catenin (CTNND1), plakoglobin (JUP), and connexin-43 (GJA1). Additionally, SRC participates in PDGF-mediated tyrosine phosphorylation of STAT1 and STAT3, the RAS pathway, EGF-mediated calcium-activated chloride channel activation, and beta-arrestin desensitization. It also plays a crucial role in EGFR internalization, CDK20/MAPK3 mitogen-activated protein kinase cascade stimulation, and osteoclastic bone resorption. SRC's multifaceted activities highlight its intricate role in cellular regulation and signal transduction.

Free

P12931

Predicted band size: 60 kDa; Observed band size: 60 kDa

Protein A affinity purified.

Cell membrane, Mitochondrion inner membrane, Nucleus, Cytoplasm.

Non-conjugated

Phosphorylated

AB_3102587

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(20μg), Calyculin A treated (HY-18983, 100ng/mL, 2hrs) as indicated using Phospho-Src (Tyr419) (HY-P80514) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Flow cytometric analysis of 1X10^6 Hela cells labeling Phospho-Src (Tyr419) Antibody(HY-P80514, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

WB, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr419 of Human Src.AA range:401-450.