NCOA4 Antibody (YA4630)

Cat. No.: HY-P84934: Data Sheet COA
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NCOA4 Antibody (YA4630) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCOA4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

NCOA4 Antibody (YA4630) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCOA4.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 70 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13772

Gene ID

8031 [NCBI]

Immunogen

Recombinant protein within human NCOA4 aa 1-400 / 614.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis (PubMed:25327288, PubMed:26436293). Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy (PubMed:25327288, PubMed:26436293). Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed:25327288). Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation (PubMed:26436293). In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma (PubMed:10347167, PubMed:8643607). Another study shows only weak behavior as a coactivator for the androgen receptor and no alteration of the ligand responsiveness of the AR (PubMed:10517667). Binds to DNA replication origins, binding is not restricted to sites of active transcription and may likely be independent from the nuclear receptor transcriptional coactivator function (PubMed:24910095). May inhibit activation of DNA replication origins, possibly by obstructing DNA unwinding via interaction with the MCM2-7 complex (PubMed:24910095)

Subcellular Localization：Cytoplasmic vesicle, autophagosome; Autolysosome; Nucleus; Chromosome

Expression：
Tissue_specificity:Widely expressed. Detected in adipose tissue and various cell lines. The β isoform is expressed only in the testes.

Induction:Down-regulated by nutrient deprivation (at protein level)

Positive sample: HeLa cell lysate, A-172 cell lysate, A549 cell lysate, A431 cell lysate, HL-60 cell lysate, NIH:OVCAR-3 cell lysate, A375 cell lysate.

Subunit：Interacts with the ferritin complex, an oligomeric structure composed of varying combinations of ferritin heavy chains and ferritin light chains (PubMed:25327288). Interacts (via C-terminus) with ferritin heavy chain FTH1 (via 'Arg-23'); thereby targeting the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed:25327288, PubMed:26436293). Interacts with ferritin light chain FTL (PubMed:25327288). Interacts (via C-terminus) with ATG8-like proteins GABARAP and GABARAPL1; thereby mediating localization to autophagosomes and ferritinophagy (By similarity). Interacts with MAP1LC3C/LC3C and GABARAPL2 (By similarity). Interacts with the androgen receptor AR (via NR DNA binding domain and NR LBD) in a ligand-dependent manner (PubMed:10517667, PubMed:15563469, PubMed:8643607). Interacts with the retinoid acid receptor RXR-gamma (RXRG) in a ligand-dependent manner (PubMed:10347167). Interacts (via N-terminus) with MCM7 (PubMed:24910095). Interacts with MCM3 (PubMed:24910095). Interacts with CDC45 (PubMed:24910095)

Database

Entrez Gene: 8031 Human

SwissProt: Q13772 Human

OMIM: 601984 Human

Synonyms

70 kDa androgen receptor activator antibody; 70 kDa androgen receptor coactivator antibody; 70 kDa AR activator antibody; 70 kDa AR-activator antibody; Androgen receptor coactivator 70 kD antibody; Androgen receptor coactivator 70 kDa protein antibody; Androgen receptor-associated protein of 70 kDa antibody; ARA 70 antibody; ARA70 antibody; DKFZp762E1112 antibody; ELE 1 antibody; ELE1 antibody; ELE1/ret TK antibody; NCOA 4 antibody; NCoA-4 antibody; NCOA4 antibody; NCOA4_HUMAN antibody; Nuclear receptor coactivator 4 antibody; Papillary thyroid carcinoma 3 antibody; PTC 3 antibody; PTC3 antibody; RET activating gene ELE1 antibody; Ret activating protein ELE1 antibody; Ret-activating protein ELE1 antibody; RFG antibody

Documentation

Select Batch:

Data Sheet (234 KB) SDS (253 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)