MCU Antibody (YA704)

MCU Antibody (YA704) is a Mouse-derived and non-conjugated IgM monoclonal antibody, targeting to MCU.

MCU protein, a mitochondrial inner membrane calcium uniporter, serves as the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex), mediating the uptake of calcium into mitochondria. Its activity is intricately regulated by MICU1 and MICU2, with low Ca(2+) levels down-regulating MCU activity and higher Ca(2+) levels stimulating MCU activity. This regulatory mechanism plays a crucial role in mitochondrial calcium homeostasis, influencing cellular physiology, bioenergetics, and various cellular signaling pathways. In cardiomyocytes, MCU participates in buffering systolic calcium rises, contributing to cardiac function. Additionally, MCU plays a key role in acute stress responses, facilitating a rapid increase in mitochondrial calcium in pacemaker cells. It is involved in processes such as mitochondrial permeability transition during ischemia-reperfusion injury, regulation of glucose-dependent insulin secretion in pancreatic beta-cells, modulation of muscle size in adults, and influencing synaptic vesicle endocytosis kinetics in central nerve terminals. The activity of MCU is inhibited by compounds like ruthenium red or its derivative Ru360.

Free

Q8NE86

Predicted band size: 40 kDa, Observed band size: 35 kDa

Protein G affinity purified.

Mitochondrion.

Non-conjugated

Unmodified

AB_3102354

Cardiovascular

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

WB: 1:500-1:1,000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane 5(40μg)), using MCU (HY-P80216) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80216, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, FC

Liquid

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgM

Endogenous

Synthetic peptide corresponding to Human MCU.AA range:51-325.