LC3B Antibody (YA308)

LC3B Antibody (YA308) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LC3B.

LC3B is a ubiquitin-like modifier involved in the formation of autophagosomal vacuoles (autophagosomes). It plays a role in mitophagy, regulating mitochondrial quantity and quality by eliminating mitochondria to a basal level to meet cellular energy demands and prevent excessive ROS production. Under cellular stress and mitochondrial fission, LC3B binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to remove damaged mitochondria. Unlike LC3s, which contribute to phagophore membrane elongation, the GABARAP/GATE-16 subfamily is essential for later stages of autophagosome maturation. LC3B also promotes primary ciliogenesis by removing OFD1 from centriolar satellites via autophagy. Through interaction with the reticulophagy receptor TEX264, LC3B participates in remodeling endoplasmic reticulum subdomains into autophagosomes during nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Under nutrient stress, LC3B directly recruits cofactor JMY to phagophore membrane surfaces, enhancing JMY's actin nucleation activity and autophagosome biogenesis during autophagy.

Free

Q9GZQ8

Predicted band size: 15 kDa; Observed band size: 14/16 kDa

Protein A affinity purified.

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion.

Non-conjugated

Unmodified

AB_3102352

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:5,000; IF-Cell: 1:100-1:200; IHC-P:1:1,000-1:5,000; IF-Tissue: 1:500-1:1,000; mIHC: 1:100; IP: Use at an assay dependent concentration

• 
  Western blot analysis of extracts from HCT116 (lane 2(20μg), C6 (lane 3(20μg) and U87 (lane 4(20μg) using LC3B (HY-P80209) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, IP, IF-Tissue, mIHC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human LC3B.AA range:1-20 &AA range:80-120.