LC3B Antibody (YA308)

LC3B Antibody (YA308) is a non-conjugated and Rabbit origined monoclonal antibody about 14/16 kDa, targeting to LC3B. It can be used for WB, IF-Cell, IHC-P, IF-Tissue, IP, mIHC assays with tag free, in the background of Human,Mouse, Rat.

Free

Q9GZQ8

Predicted band size: 15 kDa; Observed band size: 14/16 kDa

Protein A affinity purified.

Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule, Mitochondrion.

Non-conjugated

Unmodified

AB_3102352

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:5,000; IF-Cell: 1:100-1:200; IHC-P:1:1,000-1:5,000; IF-Tissue: 1:500-1:1,000; mIHC: 1:100; IP: Use at an assay dependent concentration

• 
  Western blot analysis of extracts from HCT116 (lane 2(20μg), C6 (lane 3(20μg) and U87 (lane 4(20μg) using LC3B (HY-P80209) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P, ICC/IF, IP, IF-Tissue, mIHC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human LC3B.AA range:1-20 &AA range:80-120.