IDH1 Antibody (YA3516)

IDH1 Antibody (YA3516) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IDH1.

IDH1 protein is a key enzyme that catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), a vital step in cellular metabolism and a process required by other enzymes such as the phytanoyl-CoA dioxygenase. The enzymatic activity of IDH1 is crucial for the generation of NADPH, a critical cofactor involved in various biosynthesis pathways. Additionally, IDH1 may have a distinct role as a corneal epithelial crystallin, potentially contributing to the maintenance of corneal epithelial transparency. These multifaceted functions highlight the importance of IDH1 in fundamental cellular processes and suggest its involvement in maintaining specific tissue characteristics.

Virus

O75874

Predicted band size: 47 kDa; Observed band size: 47 kDa

Non-conjugated

Unmodified

AB_3102017

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IP: 1:20

• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IDH1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, IP

Liquid

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human IDH1.The exact sequence is proprietary to MCE.