Hsp90 beta Antibody (YA355)

Cat. No.: HY-P80187: Data Sheet COA
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User Guide for Antibodies
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Hsp90 beta Antibody (YA355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp90 beta.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Hsp90 beta Antibody (YA355) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Hsp90 beta Antibody (YA355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Hsp90 beta.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 83 kDa;

Observed band size: 95 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P08238

Gene ID

3326 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Hsp90 beta.AA range:485-724.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	Use at an assay dependent concentration.

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HEK293(lane 2(20μg), Hela(lane 3(20μg), K562(lane 4(20μg) ) ,RAW264.7(lane 5(20μg) ) using Hsp90 beta (HY-P80187) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80187, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478

Subcellular Localization：Cytoplasm; Melanosome; Nucleus; Secreted; Cell membrane; Dynein axonemal particle; Cell surface

RRID

AB_3102098

Database

Entrez Gene: 3326 Human ; 15516 Mouse ; 301252 Rat

SwissProt: P08238 Human ; P11499 Mouse ; P34058 Rat

OMIM: 140572 Human

Research Field

Signal Transduction

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Hsp90 beta Antibody (YA355) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Hsp90 beta Antibody (YA355)

Hsp90 beta Antibody (YA355) Featured Recommendations:

Product Detail

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Background

Documentation

Hsp90 beta Antibody (YA355) Related Classifications

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