CD44 Antibody (YA534)

CD44 Antibody (YA534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD44.

CD44, a cell-surface receptor, plays a pivotal role in cell-cell interactions, adhesion, and migration, enabling cells to sense and respond to alterations in the tissue microenvironment. Its involvement extends to diverse cellular functions, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and response to bacterial infection. CD44 engages various extracellular matrix components, such as hyaluronan/HA, collagen, growth factors, cytokines, or proteases through its ectodomain, serving as a platform for signal transduction. This involves the assembly of protein complexes via its cytoplasmic domain, containing receptor kinases and membrane proteases. Effectors like PKN2, RAC1, RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways with CD44, leading to calcium mobilization and actin-mediated cytoskeleton reorganization, crucial for cell migration and adhesion. CD44 interacts with a spectrum of molecules, including PKN2, TIAM1, TIAM2, hyaluronan, collagen, laminin, fibronectin, UNC119, PDPN, RDX, EZR, MSN, EGFR, and CD74, forming a complex network essential for its diverse cellular functions.

Free

P16070

Predicted band size: 82 kDa;Observed band size: 75-82 kDa

Protein A affinity purified.

Cell membrane.

Non-conjugated

Unmodified

AB_3102288

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:1000-1:5000; IHC-P: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela (lane 2(20μg), MDA-MB-231 (lane 3(20μg), A549 (lane 4(20μg) using CD44 (HY-P80062) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80062, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, FC

Liquid

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human CD44.AA range:150-190.