6X His-tag Antibody (YA3432)

Cat. No.: HY-P83697: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

6X His-tag Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to 6X His-tag.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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6X His-tag Antibody (YA3432) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

6X His-tag Antibody is a non-conjugated and Rabbit origined IgG monoclonal antibody, targeting to 6X His-tag.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molecular Weight

Predicted band size: 30 kDa;

Observed band size: 32 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Species independent

Immunogen

Synthetic peptide immune sequence is HHHHHHHHC.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:5000
FC FC: Flow Cytometry	1:200-500
IP IP: Immunoprecipitation	1-2μg/mL

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HY-P70499 (lane 2(50 ng), HY-P72468 (lane 3(50 ng), HY-P7993A (lane 4(50 ng) and HY-P70620 (lane 5(50 ng) using 6X His-tag (C-terminal) (HY-P83697) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Synonyms

6 His epitope tag antibody; Hexa His tag antibody; HHHHHH epitope tag antibody; HHHHHH tag antibody; His tag antibody; Polyhistidine Tag antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

6X His-tag Antibody (YA3432) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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6X His-tag Antibody (YA3432)

6X His-tag Antibody (YA3432) Featured Recommendations:

Product Detail

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Background

Documentation

6X His-tag Antibody (YA3432) Related Classifications

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