Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11)

Cat. No.: HY-P990823: Data Sheet
FAQs
Technical Support

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) is rat-derived IgG2b κ type antibody inhibitor, targeting to PD-L1/B7-H1. Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) can block PD-L1/ B7-1 interactions and does not block PD-L1/PD-1 interactions. Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) can be used for the researches of cancer, infection, immunology and metabolic disease, such as MB49 tumor, heart graft and diabetes.

For research use only. We do not sell to patients.

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) Chemical Structure

Size		Stock
MOQ		Minimum order quantity
1 mg		Get quote
5 mg		Get quote
10 mg		Get quote
Other size		Get quote

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) Featured Recommendations:

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Isotype

Rat IgG2b kappa

Recommend Isotype Controls

Rat IgG2b kappa, Isotype Control

Species Reactivity

Mouse

IC₅₀ & Target

PD-L1/B7-H1

In Vitro

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (3 h) does not affect T cell proliferation in CD4⁺ and CD8⁺ T cells from PD-1A transgenic mice^[2].
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (50 μg/mL, 1 h) reduces replication of herpes simplex virus (HSV)-CD80 in dendritic cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (5 mg/kg, every 3 days for 4 times) does not inhibit tumor growth in MB49 tumor mice^[1].
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (0.5 mg at day 0 and 0.25 mg at days 2, 4, 6, 8 and 10, i.p.) induces diabetes in not obese diabetes of autoimmune diabetes mice^[2].
Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) (0.5 mg at day 0 and 0.25 mg at days 2, 4, 6 and 8, i.p.) aggravates cardiac allograft vasculopathy and accelerates allograft rejection in bm12 hearts transferred PD1-deficient mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Not obese diabetes of autoimmune diabetes mice(6-7 weeks or 13 weeks)^[1]
Dosage:	0.5 mg at day 0 and 0.25 mg at days 2, 4, 6, 8 and 10
Administration:	Intraperitoneally injection
Result:	Led to diabetes in 40% of 6-7-week mice over a 14 day period. Led to diabetes in 80% of 13-week mice over a two-week period. Showed marked infiltrates in pancreata.

Animal Model:	Bm12 hearts transferred PD1-deficient mice^[3]
Dosage:	0.5 mg at day 0 and 0.25 mg at days 2, 4, 6 and 8
Administration:	Intraperitoneally injection
Result:	Showed median survival time of 10 days. Increased the frequency of alloreactive T cells producing IFN-γ. Enhanced Th1 and Th2 alloimmune responses. Decreased regulatory T cell.

Gene ID

60533 [NCBI]

Accession

Q9EP73

Application

ELISA, FACS, Functional assay, Research in vivo

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Molecular Weight

150 kDa

SMILES

[Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11)]

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Inhibitory Antibodies User Guide (602 KB)

References

[1]. Po Y. Ho, et al. PD-L1-directed ISACs target host immune cells to drive powerful antitumor immune

[2]. Paterson AM, et al. The programmed death-1 ligand 1:B7-1 pathway restrains diabetogenic effector T cells in vivo. J Immunol. 2011 Aug 1;187(3):1097-105. [Content Brief]

[3]. Yang J, et al. The novel costimulatory programmed death ligand 1/B7.1 pathway is functional in inhibiting alloimmune responses in vivo. J Immunol. 2011 Aug 1;187(3):1113-9. [Content Brief]

[4]. Mott KR, et al. Inclusion of CD80 in HSV targets the recombinant virus to PD-L1 on DCs and allows productive infection and robust immune responses. PLoS One. 2014 Jan 27;9(1):e87617. [Content Brief]

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11) Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse PD-L1/B7-H1 Antibody (10F.2H11)PD-1/PD-L1PD-1/Programmed death-ligand 1PD-L1PD-1B7-H1,B7-1T cellMB49GraftDiabetesHSVInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *