2. Immunology/Inflammation MAPK/ERK Pathway Cell Cycle/DNA Damage NF-κB
  3. Interleukin Related NO Synthase p38 MAPK PERK NF-κB
  4. (±)-Stylopine

(±)-Stylopine  (Synonyms: Tetrahydrocoptisine)

Cat. No.: HY-N0924 Purity: 99.86%
COA
SDS
Handling Instructions Technical Support

(±)-Stylopine (Tetrahydrocoptisine) is an alkaloid compound. (±)-Stylopine can be isolated from the tubers of the plant Corydalis. (±)-Stylopine inhibits TNF-α, IL-6, and NO production, and attenuates phosphorylation of p38 MAPK, ERK1/2. (±)-Stylopine inhibits NF-κB expression. (±)-Stylopine exhibits anti-inflammatory activity. (±)-Stylopine has protective effects against foot edema, gastric ulcers, anxiety, depression, and acute lung injury.

For research use only. We do not sell to patients.

(±)-Stylopine

(±)-Stylopine Chemical Structure

CAS No. : 4312-32-7

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (±)-Stylopine:

(±)-Stylopine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE (±)-Stylopine

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

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nNOS iNOS eNOS

View All p38 MAPK Isoform Specific Products:

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

(±)-Stylopine (Tetrahydrocoptisine) is an alkaloid compound. (±)-Stylopine can be isolated from the tubers of the plant Corydalis. (±)-Stylopine inhibits TNF-α, IL-6, and NO production, and attenuates phosphorylation of p38 MAPK, ERK1/2. (±)-Stylopine inhibits NF-κB expression. (±)-Stylopine exhibits anti-inflammatory activity. (±)-Stylopine has protective effects against foot edema, gastric ulcers, anxiety, depression, and acute lung injury[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
GES1 IC50
> 150 μM
Compound: 1
Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay
[PMID: 35286954]
HGC-27 IC50
55.06 μM
Compound: 1
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay
[PMID: 35286954]
MGC-803 IC50
107.3 μM
Compound: 1
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay
[PMID: 35286954]
In Vitro

(±)-Stylopine (10-4 -1 μg/mL; 24 h pretreatment before 12 h LPS stimulation) significantly inhibits LPS-induced TNF-α, IL-6 production[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(±)-Stylopine Related Antibodies

Western Blot Analysis[1]

Cell Line: Mouse peritoneal macrophages
Concentration: 10-4 μg/mL, 10-2 μg/mL, 1 μg/mL
Incubation Time: 24 h
Result: Reduced phosphorylation of p38MAPK and ERK1/2.

Real Time qPCR[1]

Cell Line: Mouse peritoneal macrophages
Concentration: 0-4 μg/mL, 10-3 μg/mL, 10-2 μg/mL, 10-1 μg/mL, 1 μg/mL
Incubation Time: 24 h
Result: Downregulated TNF-α and IL-6 mRNA.
In Vivo

(±)-Stylopine (7-21 mg/kg; i.p.; 30 min before modeling) inhibits Carrageenan (HY-125474)-induced rat paw edema and reduces TNF-α, IL-6 levels in paw tissue[1].
(±)-Stylopine (10-20 mg/kg; i.p.; 3 days) attenuates ethanol-induced gastric ulcer in mice, reduces NO, TNF-α, IL-6 levels, MPO activity and NF-κB expression in gastric tissue and serum[2].
(±)-Stylopine (18.4-36.8 mg/kg; i.p.; 14 days) alleviates LPS-induced neuroinflammation in mice, improves anxiety, depression and anhedonia, reduces neurodegeneration, inhibits KMO expression, NO and lipid peroxidation[3].
(±)-Stylopine (10-20 mg/kg; i.p.; 30 min before LPS injection) reduces mortality, ameliorates lung injury, inhibits inflammatory cell infiltration, protein leakage, TNF-α, IL-6 production and NF-κB activation in LPS-induced acute lung injury in rats[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (220-250 g) with Carrageenan-induced paw edema; Male Kunming mice (20-25 g) with Xylene-induced ear edema and LPS-induced systemic inflammation[1]
Dosage: 7 mg/kg, 21 mg/kg (for Carrageenan); 10 mg/kg, 30 mg/kg (for Xylene); 10 mg/kg, 20 mg/kg, 30 mg/kg (for LPS)
Administration: i.p., 30 min before Carrageenan injection (for Carrageenan); i.p. (for Xylene); i.p., at 12 h and 1 h before LPS injection (for LPS)
Result: Reduced paw edema (56.25% and 75.35% inhibition at 5 h) and TNF-α, IL-6 in paw tissue.
Decreased ear edema weight.
Inhibited serum TNF-α in a dose- and time-dependent manner.
Molecular Weight

323.34

Formula

C19H17NO4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C1(C(CCN2CC3=C4OCOC4=CC=C3CC21)=C5)=CC6=C5OCO6
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMF : 4 mg/mL (12.37 mM; ultrasonic and warming and heat to 60°C)

DMSO : 2.5 mg/mL (7.73 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Acetone : 1 mg/mL (3.09 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0927 mL 15.4636 mL 30.9272 mL
5 mM 0.6185 mL 3.0927 mL 6.1854 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  20% HP-β-CD in Saline

    Solubility: 12.5 mg/mL (38.66 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.86%

SDS (393 KB)

COA (252 KB) HNMR (248 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Acetone / DMSO / DMF 1 mM 3.0927 mL 15.4636 mL 30.9272 mL 77.3180 mL
DMSO / DMF 5 mM 0.6185 mL 3.0927 mL 6.1854 mL 15.4636 mL
DMF 10 mM 0.3093 mL 1.5464 mL 3.0927 mL 7.7318 mL
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(±)-Stylopine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-Stylopine4312-32-7TetrahydrocoptisineInterleukin RelatedNO Synthasep38 MAPKPERKNF-κBILNitric oxide synthasesNOSProtein kinase R-like endoplasmic reticulum kinasePKR-like endoplasmic reticulum kinaseNuclear factor-κBNuclear factor-kappaBCorydalisanti-parasiticalkaloidInhibitorinhibitorinhibit

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