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  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-100575
    Acriflavine
    		98.0%
    Acriflavine (Acriflavinium chloride) is a fluorescent acridine dye that can be used to label nucleic acid. Acriflavine is an antiseptic agent. Acriflavine is a potent HIF-1 inhibitor that prevents the dimerization of HIF-1α and HIF-1β subunits. Acriflavine inhibits the interaction between monocarboxylate transporter 4 (MCT4) and Basigin. Acriflavine is used in cancer research, such as breast cancer, brain tumor and chronic myeloid leukemia. Acriflavine is a potent papain-like protease (PLpro) inhibitor, which inhibits SARS-CoV-2.
    Acriflavine
  • HY-D1670
    Z-Gly-Pro-AMC
    		99.80%
    Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm).
    Z-Gly-Pro-AMC
  • HY-D1594
    BODIPY TR Cadaverine
    		99.05%
    BODIPY TR Cadaverine, a cadaverine derivative, is a red fluorescent dye. BODIPY TR Cadaverine can be used in a a highly sensitive and robust fluorescent displacement assay, which binds to native LPS strongly, specifically recognizing lipid A, and is competitively displaced by compounds displaying an affinity for lipid A.
    BODIPY TR Cadaverine
  • HY-D1775
    LysoTracker Blue DND-22
    LysoTracker blue DND-22 is a blue fluorescent dye (Ex/Em: 373/422 nm). LysoTracker blue DND-22 stains acidic regions in living cells. LysoTracker blue DND-22 is used in the researches of neurodegenerative diseases and leukemia.
    LysoTracker Blue DND-22
  • HY-D0915
    Brilliant Blue FCF
    		≥98.0%
    Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.
    Brilliant Blue FCF
  • HY-101891
    Di-8-ANEPPS
    		99.62%
    Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .
    Di-8-ANEPPS
  • HY-P1003
    Ac-DEVD-AMC
    		99.93%
    Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm.
    Ac-DEVD-AMC
  • HY-B1335
    Pentetic acid
    		98.0%
    Pentetic acid (Diethylenetriaminepentaacetic acid) is an orally active compound with biodegradability used to construct magnetic adsorbent, which can simultaneously remove heavy metal and dye from complex wastewater. Pentetic acid can form strong metal complexes, which prevents metal ions from catalysing the decomposition of peroxygen chemicals, especially hydrogen peroxide.
    Pentetic acid
  • HY-D0716
    Fluo-3AM
    		≥99.0%
    Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
    Fluo-3AM
  • HY-D1738
    DAPI dilactate
    		99.0%
    DAPI (dilactate) is a blue fluorescent dye that preferentially binds dsDNA and binds to minor groove AT clusters. DAPI (dilactate) is combined with dsDNA, and the fluorescence was enhanced about 20-fold. DAPI (dilactate) can be used to identify the cell cycle and specifically stains the nucleus but not the cytoplasm. DAPI (dilactate) form is more soluble in water than DAPI (dihydrochloride) form.
    DAPI dilactate
  • HY-D0832
    CY5-N3
    		98.07%
    CY5-N3 (Sulfo-Cyanine5-azide) is a Cy5-azide, which is a fluorescent dye (ex/em: 646/662 nm). CY5-N3 is cell membrane permeable and can be used for live cell imaging. CY5-N3 can be used in cell imagine by Click reaction.
    CY5-N3
  • HY-D0819A
    CY5-SE triethylamine salt
    		98.0%
    Cy5-SE (Cy5 NHS Ester) triethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Cy5-SE triethylamine salt is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides Ex=649 nm; Em=670 nm).
    CY5-SE triethylamine salt
  • HY-D0001
    Alcian Blue 8GX
    Alcian Blue 8GX is a commonly used phthalocyanine dye that binds to glycoproteins and glycosaminoglycans. Alcian Blue 8GX has a wide range of applications in biological staining, including proteins in brain tumors and DNA in cells and tissues.
    Alcian Blue 8GX
  • HY-D1805
    Vari Fluor 647-Streptavidin
    Vari Fluor 647-Streptavidin is a dye marker of Vari Fluor-streptavidin consisting of labeling streptavidin with a Vari Fluor series of fluorescent probes. Streptavidin is a high-affinity tetramer protein, each tetramer consisting of four identical streptavidin subunits. Streptavidin binds to biotin specifically via a reversible non-covalent effect. Streptavidin can achieve rapid and efficient detection of biotin markers, and is often used in immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunohistochemical staining (IFH), in situ hybridization (ISH) and other experiments. Ex/Em=650 nm/665 nm.
    Vari Fluor 647-Streptavidin
  • HY-123645
    Rhodamine B hydrazide
    		99.87%
    Rhodamine B hydrazide is a fluorescent derivative based on rhodamine B, containing the spirocyclic structure of Rhodamine B (HY-Y0016), which can be used to detect copper ions (Cu2+), mercury ions, peroxynitrite, hydroxyl radicals and nitric oxide (NO).
    Excitation/emission wavelength:
    Conventional detection: 510/578 nm.
    Sulfite detection: 554 nm absorption, 574 nm emission (due to the formation of Rhodamine B fluorescent product).
    Rhodamine B hydrazide
  • HY-D1068
    Cy5-DBCO
    		99.86%
    Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR)red fluorescent dye with λabsand λem of 646nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background. Cy5-DBCO is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
    Cy5-DBCO
  • HY-135985
    DCLK1-IN-1
    		99.35%
    DCLK1-IN-1 is a selective, oral bioavailability in vivo-compatible chemical probe of the doublecortin like kinase 1 (DCLK1 kinase) domain. DCLK1-IN-1 inhibits DCLK1 and DCLK2 kinases (IC50: DCLK1=9.5/57.2 nM and DCLK2=31/103 nM in binding and kinase assay, respectively). DCLK1-IN-1 shows low toxicity, and can investigate DCLK1 biology and establish its role in cancer, like DCLK1+ pancreatic ductal adenocarcinoma (PDAC).
    DCLK1-IN-1
  • HY-D1449
    IR-1061
    IR-1061 is a near infrared fluorescent organic dye for in vivo bimodal imaging.
    IR-1061
  • HY-D0169
    Phenol Red
    		98.0%
    Phenol red (Phenolsulfonephthalein) is a pH indicator dye, relying on a distinct color change from pink to yellow in case of a positive reaction .
    Phenol Red
  • HY-D0236
    Congo Red
    Congo Red is an azo dye. Congo Red (CR) binding been used as a diagnostic test for the presence of amyloid in tissue sections.
    Congo Red
Cat. No. Product Name / Synonyms Application Reactivity