LysoTracker Blue DND-22 

LysoTracker blue DND-22 is a blue fluorescent dye (Ex/Em: 373/422 nm). LysoTracker blue DND-22 stains acidic regions in living cells. LysoTracker blue DND-22 is used in the researches of neurodegenerative diseases and leukemia^[1][2].

Guidelines (The following is our recommended protocol, which is provided as a guide only and should be modified to suit your specific needs).
Staining steps^[2]
1) Under the conditions of 37°C and 5% CO₂, U937 cells are cultured in IMDM medium containing 10% fetal bovine serum (HY-T1000), 100 units/mL Penicillin, and 50 μg/mL Streptomycin (HY-B1906).
2) Cells are treated with labeled oxLDL (18 μg/mL) or oxLDL-IC (24 μg/mL).
3) 50 nM LysoTracker Blue DND-22 is added to the cells 30 min before the end of the incubation with labeled oxLDL and oxLDL-IC.
4) After the incubation, cells are washed three times with serum-free IMDM to remove unbound dye.
5) Cells are suspended in 100 μL of IMDM, and then 10 μL aliquots are loaded into glass capillaries and sealed.
6) Cells are visualized using a confocal microscope with an excitation wavelength of 373 nm and an emission wavelength of 422 nm to observe lysosomal localization.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

524.40

C₂₄H₃₈Cl₄N₄

215247-93-1

Solid

Light yellow to yellow

CN(CCNCC1=C2C=CC=CC2=C(CNCCN(C)C)C3=CC=CC=C31)C.Cl.Cl.Cl.Cl

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Zeng J, et al. Biodegradable PLGA nanoparticles restore lysosomal acidity and protect neural PC-12 cells against mitochondrial toxicity. Ind Eng Chem Res. 2019 Aug 7;58(31):13910-13917.  [Content Brief]

  [2]. Al Gadban MM, et al. Differential trafficking of oxidized LDL and oxidized LDL immune complexes in macrophages: impact on oxidative stress. PLoS One. 2010 Sep 2;5(9):e12534.  [Content Brief]