2. NF-κB Immunology/Inflammation Stem Cell/Wnt
  3. RANKL/RANK Toll-like Receptor (TLR) Wnt β-catenin
  4. Pamidronate disodium pentahydrate

Pamidronate disodium pentahydrate, the second-generation nitrogen-containing bisphosphonate, is an inhibitor of bone loss. Pamidronate disodium pentahydrate significantly inhibits subchondral bone loss in early osteoarthritis by upregulating the expression of osteoprotegerin in cartilage and subchondral bone, and inhibiting the expression of RANKL and MMP-9 in both tissues, as well as TLR-4 in cartilage, thereby alleviating cartilage degeneration. Additionally, Pamidronate disodium pentahydrate can inhibit the signaling of Wnt and β-catenin, and is applicable for research on osteoporosis and osteosarcoma.

For research use only. We do not sell to patients.

Pamidronate disodium pentahydrate Chemical Structure

Pamidronate disodium pentahydrate Chemical Structure

CAS No. : 109552-15-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

Other Forms of Pamidronate disodium pentahydrate:

Pamidronate disodium pentahydrate Related Antibodies

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1 Publications Citing Use of MCE Pamidronate disodium pentahydrate

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  • Biological Activity

  • Purity & Documentation

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Description

Pamidronate disodium pentahydrate, the second-generation nitrogen-containing bisphosphonate, is an inhibitor of bone loss. Pamidronate disodium pentahydrate significantly inhibits subchondral bone loss in early osteoarthritis by upregulating the expression of osteoprotegerin in cartilage and subchondral bone, and inhibiting the expression of RANKL and MMP-9 in both tissues, as well as TLR-4 in cartilage, thereby alleviating cartilage degeneration. Additionally, Pamidronate disodium pentahydrate can inhibit the signaling of Wnt and β-catenin, and is applicable for research on osteoporosis and osteosarcoma[1][2][3][4].

In Vitro

Pamidronate disodium pentahydrate (0.001-1000 µM, 72 h) inhibits the cell viability of osteosarcoma cells POS, HMPOS and COS31, exhibits no cytotoxicity to fibroblasts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pamidronate disodium pentahydrate Related Antibodies

Cell Viability Assay[1]

Cell Line: POS, HMPOS and COS31
Concentration: 0.001-1000 µM
Incubation Time: 72 h
Result: Inhibited the cell viability.
In Vivo

Pamidronate disodium pentahydrate (3 mg/kg, ear vein injection, once monthly for 14 weeks) improves the bone structure, alleviates the degenerative changes of cartilage, exhibits protective effect against osteoarthritis in rabbit models[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Anterior cruciate ligament transection (ACLT)-induced osteoarthritis in rabbits[3]
Dosage: 3 mg/kg
Administration: ear vein injection, once monthly for 14 weeks
Result: Increased bone volume fraction (BV/TV), trabecular thickness (TbTh) and trabecular number (TbN).
Reduced the erosion of cartilage surface, increase the content of chondrocytes and matrix.
Clinical Trial
Molecular Weight

369.11

Formula

C3H19NNa2O12P2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OC(P(O)(O[Na])=O)(CCN)P(O)(O[Na])=O.O.O.O.O.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 7.14 mg/mL (19.34 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7092 mL 13.5461 mL 27.0922 mL
5 mM 0.5418 mL 2.7092 mL 5.4184 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 20 mg/mL (54.18 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.7092 mL 13.5461 mL 27.0922 mL 67.7305 mL
5 mM 0.5418 mL 2.7092 mL 5.4184 mL 13.5461 mL
10 mM 0.2709 mL 1.3546 mL 2.7092 mL 6.7730 mL
15 mM 0.1806 mL 0.9031 mL 1.8061 mL 4.5154 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pamidronate disodium pentahydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pamidronate disodium109552-15-0RANKL/RANKToll-like Receptor (TLR)Wntβ-cateninBeta cateninInhibitorinhibitorinhibit

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