2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Neuronal Signaling Membrane Transporter/Ion Channel Apoptosis
  3. Reactive Oxygen Species Calcium Channel Apoptosis Endogenous Metabolite
  4. L-Ascorbic acid

L-Ascorbic acid  (Synonyms: L-Ascorbate; Vitamin C)

Cat. No.: HY-B0166 Purity: 99.97%
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L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Cav3.2 channels with an IC50 of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells.

For research use only. We do not sell to patients.

L-Ascorbic acid Chemical Structure

L-Ascorbic acid Chemical Structure

CAS No. : 50-81-7

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Based on 63 publication(s) in Google Scholar

Other Forms of L-Ascorbic acid:

L-Ascorbic acid Related Antibodies

Top Publications Citing Use of Products

57 Publications Citing Use of MCE L-Ascorbic acid

Cell Viability Assay
WB
IHC

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 May 27.

    L-Ascorbic acid (Vitamin C) (50-500 μM; 6 h) significantly increases the viability of heat-stressed gastric cancer cells at concentrations of 200 μM and 300 μM.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 May 31;24(1):192.  [Abstract]

    L-Ascorbic acid (AA) (150 μL of 1.5 M; i.p.; every two days) enhances the expression of GPX4 and SLC7A11 proteins in mouse liver.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2024 May 31;24(1):192.  [Abstract]

    L-Ascorbic acid (AA) (150ul of 1.5 M; i.p.; every two days) significantly decreases the expression of Ki67 in C57BL/6 mice.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Food Funct. 2022 May 10;13(9):5089-5101.  [Abstract]

    L-Ascorbic acid (Vitamin C) (100 μM; 10 min–4 h) upregulates the protein expression levels of IGF-1, p-IGF-1R, and SVCT2 in a time-dependent manner.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Cell Biol Toxicol. 2021 Jul 20.  [Abstract]

    L-Ascorbic acid (300 μM, 10 μL intrathecal injection) reduces the levels of CaV3.2, p-CaMKII, and Cx43 in mice.

    L-Ascorbic acid purchased from MedChemExpress. Usage Cited in: Antioxid Redox Signal. 2020 Dec 10;33(17):1191-1208.  [Abstract]

    L-Ascorbic acid (Vitamin C) (250 μM, 1 h) significantly alleviates the growth suppression induced by combined SAS and PAG treatment only in OVISE cells, but shows no effect in KOC-7C cells.

    View All Calcium Channel Isoform Specific Products:

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    N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    L-Ascorbic acid (L-Ascorbate), an electron donor, is an endogenous antioxidant agent. L-Ascorbic acid inhibits selectively Cav3.2 channels with an IC50 of 6.5 μM. L-Ascorbic acid is also a collagen deposition enhancer and an elastogenesis inhibitor[1][2][3]. L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[4].

    IC50 & Target

    T-type calcium channel

     

    Microbial Metabolite

     

    In Vitro

    The anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Ascorbic acid Related Antibodies

    Cell Viability Assay[4]

    Cell Line: High SVCT-2 expressing cell lines Sw620, Sw480, LoVo, SNU-C4; Low SVCT-2 expressing cell lines HCT15, HCT116, DLD-1, CoLo-205
    Concentration: 0, 0.1 μM, 1 μM, 10 μM, 0.1 mM, 0.5 mM, 1 mM, and 2 mM
    Incubation Time: 24 hours
    Result: Some high SVCT-2 expressing cancer cells demonstrated a dramatic cell-autonomous inhibitory effect of L-ascorbic acid.
    Low SVCT-2 expressing cell lines showed biphasic responses to L-ascorbic acid.

    Western Blot Analysis[4]

    Cell Line: Sw620, Sw480, LoVo, SNU-C4, HCT15, HCT116, DLD-1, CoLo-205 cell lines
    Concentration: 1 mM
    Incubation Time:
    Result: The cell lines showed different levels of SVCT-2 expression in western blot analyses: Sw620, Sw480, and Lovo expressed high levels of SVCT-2 whereas HCT116, HCT15, and DLD-1 expressed low levels.
    In Vivo

    L-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Normal rats:Albino rats of either sex weighing between 125-175 g[5]
    Dosage: Group I received L-ascorbic acid 60 mg/kg, Group II received Tolbutamide 20 mg/kg and Group III was given L-ascorbic acid (60 mg/kg) prior to the administration of tolbutamide 20 mg/kg
    Administration: Administered orally
    Result: L-ascorbic acid at the dose of 60 mg/kg produced 50.91% blood glucose reduction at 0.5 h and 20 mg/kg body weight of Tolbutamide produced 33% at 4 h as peak effects. In the presence of L-ascorbic acid (60 mg/kg), the action of Tolbutamide was early in onset and maintained for 6 h.
    Animal Model: Diabetic rats:Albino rats of either sex weighing between 125 to 175 g were fasted overnight before injection with Alloxan[5]
    Dosage: Group I received L-ascorbic acid 40 mg/kg and Group II received Tolbutamide 20 mg/kg while Group III was given L-ascorbic acid 40 mg/kg prior to Tolbutamide administration (20 mg/kg).
    Administration: Oral administration
    Result: L-ascorbic acid (40 mg/kg alone) produced 42.53% blood glucose reduction at 1.5 h and Tolbutamide 20 mg/kg produced 45.09 at 4 h. Administration of L-ascorbic acid 40 mg/kg body weight prior to Tolbutamide produced antidiabetic activity at 0.5 h and was maintained for 6 h.
    Clinical Trial
    Molecular Weight

    176.12

    Formula

    C6H8O6
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    RT, protect from light

    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (567.79 mM)

    DMSO : 100 mg/mL (567.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.6779 mL 28.3897 mL 56.7795 mL
    5 mM 1.1356 mL 5.6779 mL 11.3559 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (567.79 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
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    Dosing volume
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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.97%

    SDS (393 KB)

    COA (252 KB) RP-HPLC (205 KB) LCMS (93 KB) Elemental Analysis Report (111 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 5.6779 mL 28.3897 mL 56.7795 mL 141.9487 mL
    5 mM 1.1356 mL 5.6779 mL 11.3559 mL 28.3897 mL
    10 mM 0.5678 mL 2.8390 mL 5.6779 mL 14.1949 mL
    15 mM 0.3785 mL 1.8926 mL 3.7853 mL 9.4632 mL
    20 mM 0.2839 mL 1.4195 mL 2.8390 mL 7.0974 mL
    25 mM 0.2271 mL 1.1356 mL 2.2712 mL 5.6779 mL
    30 mM 0.1893 mL 0.9463 mL 1.8926 mL 4.7316 mL
    40 mM 0.1419 mL 0.7097 mL 1.4195 mL 3.5487 mL
    50 mM 0.1136 mL 0.5678 mL 1.1356 mL 2.8390 mL
    60 mM 0.0946 mL 0.4732 mL 0.9463 mL 2.3658 mL
    80 mM 0.0710 mL 0.3549 mL 0.7097 mL 1.7744 mL
    100 mM 0.0568 mL 0.2839 mL 0.5678 mL 1.4195 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Ascorbic acid Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Ascorbic acid50-81-7L-Ascorbate Vitamin CReactive Oxygen SpeciesCalcium ChannelApoptosisEndogenous MetaboliteCa2+ channelsCa channelsElectrondonorantioxidantCav3.2channelcollagendepositionreactiveoxygenspeciesROScancerInhibitorinhibitorinhibit

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