8-Deoxylactucin

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8-Deoxylactucin is an orally active sesquiterpene lactone. 8-Deoxylactucin inhibits LPS (HY-D1056)-induced NO production in RAW264.7 macrophages with an IC₅₀ value of 4.35 μM. 8-Deoxylactucin exerts anti-inflammatory effects by blocking the NF-κB pathway. 8-Deoxylactucin demonstrates hepatoprotective efficacy in LPS/D-galactosamine-induced acute hepatitis model of mice. 8-Deoxylactucin can be used for the study of inflammatory diseases and inflammatory liver injuries.

For research use only. We do not sell to patients.

8-Deoxylactucin Chemical Structure

CAS No. : 65725-10-2

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nNOS iNOS eNOS

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Description

In Vitro

8-Deoxylactucin (1 h pretreatment) significantly inhibits LPS (HY-D1056)-induced NO production in RAW264.7 macrophages, with an IC₅₀ of 4.35 μM^[1].
8-Deoxylactucin (1-10 μM, 1 h pretreatment) significantly reduces LPS-induced mRNA and protein expression of iNOS, as well as mRNA expression of pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α in RAW264.7 cells^[1].
8-Deoxylactucin (1-10 μM, 2 h pretreatment) significantly blocks LPS-induced phosphorylation of IKKα/β and IκBα, inhibits IκBα degradation, and reduces nuclear accumulation of NF-κB p65 in RAW264.7 macrophages^[1].
8-Deoxylactucin (1-10 μM, 1 h pretreatment) significantly enhances protein expression and nuclear translocation of NRF2, up-regulates protein expression of NRF2-targeted antioxidant genes (HO, NQO1), and reduces ROS production in RAW264.7 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:	RAW264.7 macrophages
Concentration:	1, 5, 10 μM
Incubation Time:	1, 2 h pretreatment
Result:	Reduced the mRNA expression of pro-inflammatory cytokines including IL-1β, IL-6, and TNF-α in RAW264.7 cells.

Western Blot Analysis^[1]

Cell Line:	RAW264.7 macrophages
Concentration:	1, 5, 10 μM
Incubation Time:	1, 2 h pretreatment
Result:	Reduced LPS-induced expression of iNOS. Blocked LPS-induced phosphorylation of IKKα/β and IκBα. Inhibited IκBα degradation. Reduced nuclear accumulation of NF-κB p65.

In Vivo

8-Deoxylactucin (2-10 mg/kg, i.g., once daily, 5 days) significantly reduces LPS/D-galactosamine (D-GalN)-induced increase in liver index (liver weight/body weight) in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LPS/D-galactosamine (D-GalN) (LPS: 50 μg/kg, D-GalN: 700 mg/kg, i.p. injection) was administered to 6-week-old male ICR mice (22-24 g) to induce acute hepatitis model^[1]
Dosage:	2, 5, 10 mg/kg
Administration:	i.g., once daily, 5 days
Result:	Achieved significant reduction in liver index. Showed significant alleviation of liver tissue damage and decreased pathological scores. Reduced serum ALT and AST levels. Decreased protein expression of IL-1β, IL-6, TNF-α, and 4-HNE in liver tissues.

Molecular Weight

260.29

Formula

C₁₅H₁₆O₄

CAS No.

65725-10-2

SMILES

OCC([C@]1([H])[C@]2([H])[C@](CCC(C)=C13)([H])C(C(O2)=O)=C)=CC3=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Zhou Y, et al. Sesquiterpene lactones from Cichorium intybus exhibit potent anti-inflammatory and hepatoprotective effects by repression of NF-κB and enhancement of NRF2. J Ethnopharmacol. 2025 Mar 13;343:119439. [Content Brief]

8-Deoxylactucin Related Classifications

Inflammation/Immunology

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

8-Deoxylactucin65725-10-2NF-κBNO SynthaseReactive Oxygen Species (ROS)Nuclear factor-κBNuclear factor-kappaBNitric oxide synthasesNOSCichorium intybusinflammatory diseasesNORAW264.7NF-κB pathwayInhibitorinhibitorinhibit

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