2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Acetoacetic acid

Acetoacetic acid is an oxidative stress inducer that affects the antioxidant enzyme system and lipoprotein metabolism. Acetoacetic acid induces oxidative stress by decreasing the mRNA expression and activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increasing MDA content, and inhibiting very low density lipoprotein (VLDL) assembly by downregulating apolipoprotein ApoB100, ApoE, and low density lipoprotein receptor (LDLR), leading to triglyceride (TG) accumulation in hepatocytes. Acetoacetic acid can be used to study metabolic diseases.

The free form of the compound is prone to instability, it is advisable to consider the stable salt form (Acetoacetic acid lithium) that retains the same biological activity.

For research use only. We do not sell to patients.

Acetoacetic acid Chemical Structure

Acetoacetic acid Chemical Structure

CAS No. : 541-50-4

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Acetoacetic acid Related Antibodies

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Description

Acetoacetic acid is an oxidative stress inducer that affects the antioxidant enzyme system and lipoprotein metabolism. Acetoacetic acid induces oxidative stress by decreasing the mRNA expression and activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increasing MDA content, and inhibiting very low density lipoprotein (VLDL) assembly by downregulating apolipoprotein ApoB100, ApoE, and low density lipoprotein receptor (LDLR), leading to triglyceride (TG) accumulation in hepatocytes. Acetoacetic acid can be used to study metabolic diseases[1][2][3].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

In the bovine hepatocyte experiment, Acetoacetic acid (0.6, 2.4, 4.8 mM; 24 h) dose-dependently reduces the mRNA expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increases the malondialdehyde (MDA) content, induced oxidative stress, and downregulates the mRNA expression of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and low-density lipoprotein receptor (LDLR), inhibits the assembly of very low-density lipoprotein (VLDL), and leads to the accumulation of intracellular triglycerides (TG) and a decrease in the VLDL content in the culture medium[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Acetoacetic acid Related Antibodies

RT-PCR[1]

Cell Line: Bovine hepatocytes
Concentration: 0 (control), 0.6 mM, 2.4 mM, 4.8 mM
Incubation Time: 24 h
Result: Significantly downregulated the mRNA expression of antioxidant enzymes (SOD, CAT, GSH-Px) and VLDL-related proteins (ApoB100, ApoE, LDLR) in a dose-dependent manner compared to the control group.
Increased intracellular malondialdehyde (MDA) content, a marker of oxidative stress, while the activities of SOD, CAT, and GSH-Px were reduced.
Resulted intracellular triglyceride (TG) accumulation, and significantly decreased the secretion of VLDL into the culture medium in a dose-dependent manner.
Molecular Weight

102.09

Formula

C4H6O3
CAS No.
SMILES

CC(CC(O)=O)=O
Initial Source
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Acetoacetic acid Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Acetoacetic acid541-50-4Endogenous MetaboliteInhibitorinhibitorinhibit

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