Acetoacetic acid lithium

Name: Acetoacetic acid lithium
Brand: MedChemExpress
SKU: HY-112540A
Rating: 5.0 (3 reviews)

Cat. No.: HY-112540A Purity: ≥98.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Acetoacetic acid lithium is an oxidative stress inducer that affects the antioxidant enzyme system and lipoprotein metabolism. Acetoacetic acid lithium induces oxidative stress by decreasing the mRNA expression and activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increasing MDA content, and inhibiting very low density lipoprotein (VLDL) assembly by downregulating apolipoprotein ApoB100, ApoE, and low density lipoprotein receptor (LDLR), leading to triglyceride (TG) accumulation in hepatocytes. Acetoacetic acid lithium can be used to study metabolic diseases.

For research use only. We do not sell to patients.

Acetoacetic acid lithium Chemical Structure

CAS No. : 3483-11-2

Size	Stock	Quantity
Free Sample (0.1 - 0.5 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Acetoacetic acid lithium:

Acetoacetic acid sodium In-stock
Acetoacetic acid Get quote

3 Publications Citing Use of MCE Acetoacetic acid lithium

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

In the bovine hepatocyte experiment, acetoacetic acid lithium (0.6, 2.4, 4.8 mM; 24 h) dose-dependently reduces the mRNA expression and enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), increases the malondialdehyde (MDA) content, induced oxidative stress, and downregulates the mRNA expression of apolipoprotein B100 (ApoB100), apolipoprotein E (ApoE), and low-density lipoprotein receptor (LDLR), inhibits the assembly of very low-density lipoprotein (VLDL), and leads to the accumulation of intracellular triglycerides (TG) and a decrease in the VLDL content in the culture medium^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Bovine hepatocytes
Concentration:	0 (control), 0.6 mM, 2.4 mM, 4.8 mM
Incubation Time:	24 h
Result:	Significantly downregulated the mRNA expression of antioxidant enzymes (SOD, CAT, GSH-Px) and VLDL-related proteins (ApoB100, ApoE, LDLR) in a dose-dependent manner compared to the control group. Increased intracellular malondialdehyde (MDA) content, a marker of oxidative stress, while the activities of SOD, CAT, and GSH-Px were reduced. Resulted intracellular triglyceride (TG) accumulation, and significantly decreased the secretion of VLDL into the culture medium in a dose-dependent manner.

Molecular Weight

108.02

Formula

C₄H₅LiO₃

CAS No.

3483-11-2

Appearance

Solid

Color

White to off-white

SMILES

CC(CC(O[Li])=O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (925.75 mM; Need ultrasonic)

DMSO : 1.54 mg/mL (14.26 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	9.2575 mL	46.2877 mL	92.5754 mL
5 mM	1.8515 mL	9.2575 mL	18.5151 mL
10 mM	0.9258 mL	4.6288 mL	9.2575 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (245 KB) HNMR (204 KB)

Handling Instructions (2659 KB)

References

[1]. Shi X, et al. Acetoacetic acid induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes. J Dairy Res. 2016 Nov;83(4):442-446. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	9.2575 mL	46.2877 mL	92.5755 mL	231.4386 mL
	5 mM	1.8515 mL	9.2575 mL	18.5151 mL	46.2877 mL
	10 mM	0.9258 mL	4.6288 mL	9.2575 mL	23.1439 mL
H₂O	15 mM	0.6172 mL	3.0858 mL	6.1717 mL	15.4292 mL
	20 mM	0.4629 mL	2.3144 mL	4.6288 mL	11.5719 mL
	25 mM	0.3703 mL	1.8515 mL	3.7030 mL	9.2575 mL
	30 mM	0.3086 mL	1.5429 mL	3.0858 mL	7.7146 mL
	40 mM	0.2314 mL	1.1572 mL	2.3144 mL	5.7860 mL
	50 mM	0.1852 mL	0.9258 mL	1.8515 mL	4.6288 mL
	60 mM	0.1543 mL	0.7715 mL	1.5429 mL	3.8573 mL
	80 mM	0.1157 mL	0.5786 mL	1.1572 mL	2.8930 mL
	100 mM	0.0926 mL	0.4629 mL	0.9258 mL	2.3144 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.