2. Cell Cycle/DNA Damage Anti-infection
  3. Nucleoside Antimetabolite/Analog DNA/RNA Synthesis Dengue Virus Flavivirus CHIKV SARS-CoV
  4. 2-Thiouridine

2-Thiouridine is an orally active modified nucleobase. 2-Thiouridine stabilizes U:A base pairs and destabilizes U:G wobble base pairs. 2-Thiouridine significantly improves the efficiency and accuracy of nonenzymatic replication of mixed-sequence A/U-containing RNA templates. 2-Thiouridine exhibits antiviral activity against multiple positive-sense single-stranded RNA viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ( [HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV).

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2-Thiouridine

2-Thiouridine Chemical Structure

CAS No. : 20235-78-3

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10 mM * 1 mL in DMSO
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2-Thiouridine Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

2-Thiouridine is an orally active modified nucleobase. 2-Thiouridine stabilizes U:A base pairs and destabilizes U:G wobble base pairs. 2-Thiouridine significantly improves the efficiency and accuracy of nonenzymatic replication of mixed-sequence A/U-containing RNA templates. 2-Thiouridine exhibits antiviral activity against multiple positive-sense single-stranded RNA viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ( [HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV)[1][2][3][4].

In Vitro

2-Thiouridine (0.625-40 μM) inhibits the replication of ssRNA+ viruses (DENV2, ZIKV, YFV, JEV, WNV, CHIKV, human coronaviruses ([HCoV]-229E, HCoV-OC43, SARS-CoV), and MERS-CoV) in a dose-dependent manner[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2-Thiouridine Related Antibodies
In Vivo

2-Thiouridine (50-150 mg/kg; p.o.; twice daily) prolongs the survival rate and reduces the viral RNA load in serum, spleen and other tissues in DENV2-infected mice[4].
2-Thiouridine (2-300 mg/kg; i.v. (2-20 mg/kg; once a day) or p.o. (300 mg/kg; twice a day); 5 days) improves the survival rate and reduces the lung viral titer and inflammatory factor levels in the SARS-CoV-2 infection mouse model[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

260.27

Formula

C9H12N2O5S
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

S=C(N1)N([C@@H]2O[C@H](CO)[C@@H](O)[C@H]2O)C=CC1=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (192.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 10 mg/mL (38.42 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8422 mL 19.2108 mL 38.4216 mL
5 mM 0.7684 mL 3.8422 mL 7.6843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.73%

SDS (393 KB)

COA (252 KB) HNMR (248 KB) RP-HPLC (262 KB) LCMS (95 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.8422 mL 19.2108 mL 38.4216 mL 96.0541 mL
5 mM 0.7684 mL 3.8422 mL 7.6843 mL 19.2108 mL
10 mM 0.3842 mL 1.9211 mL 3.8422 mL 9.6054 mL
15 mM 0.2561 mL 1.2807 mL 2.5614 mL 6.4036 mL
20 mM 0.1921 mL 0.9605 mL 1.9211 mL 4.8027 mL
25 mM 0.1537 mL 0.7684 mL 1.5369 mL 3.8422 mL
30 mM 0.1281 mL 0.6404 mL 1.2807 mL 3.2018 mL
DMSO 40 mM 0.0961 mL 0.4803 mL 0.9605 mL 2.4014 mL
50 mM 0.0768 mL 0.3842 mL 0.7684 mL 1.9211 mL
60 mM 0.0640 mL 0.3202 mL 0.6404 mL 1.6009 mL
80 mM 0.0480 mL 0.2401 mL 0.4803 mL 1.2007 mL
100 mM 0.0384 mL 0.1921 mL 0.3842 mL 0.9605 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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2-Thiouridine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2-Thiouridine20235-78-3Nucleoside Antimetabolite/AnalogDNA/RNA SynthesisDengue VirusFlavivirusCHIKVSARS-CoVDENVChikungunya virus SARS coronavirusindolent lymphoid malignanciesantitumorInhibitorinhibitorinhibit

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