Weigert’s Elastic Fiber Staining Kit 

Weigert’s Elastic Fiber Staining Kit can be used to stain elastic fibers. Its staining principle lies in the formation of a Lake complex between resorcinol fuchsin and iron ions. The free amino groups of this complex are firmly bonded to the hydrogen bonds within the elastic fibers, resulting in a black color.

Guidelines (The following are our recommended protocols. This protocol is for reference only and should be modified according to your specific needs.)
1. Dewaxing and Hydration
Immerse the paraffin sections in the following solutions sequentially: Xylene I: 10-15 minutes, Xylene II: 10-15 minutes, Anhydrous Ethanol I: 5 minutes, Anhydrous Ethanol II: 5 minutes, 95% Ethanol: 5 minutes, 80% Ethanol: 5 minutes. Rinse briefly with tap water: 1 minute.
2. Immerse the sections in a staining jar containing Weigert's resorcinol fuchsin solution (cover) for 1-3 hours or at 56°C for 0.5-1 hour.
3. Remove the sections and rinse slowly with tap water for approximately 30 seconds to 1 minute to remove any excess stain.
4. Remove the cytoplasmic background stain with 95% ethanol and differentiate until no staining solution remains. If differentiation is ineffective, differentiate with acidic differentiation solution for 2-3 seconds, then rinse thoroughly with water for 5-10 minutes.
5. Counterstain with VG staining solution for 30 seconds, rinse rapidly with water, and immediately differentiate and dehydrate with 95% ethanol.
6. Dehydration, clearing, and mounting: Anhydrous ethanol I: 2-3 minutes, Anhydrous ethanol II: 2-3 minutes, Xylene I: 5-10 minutes, Xylene II: 5-10 minutes. Remove the section from the xylene, wipe away excess xylene around the tissue with a paper towel (but do not allow the tissue to dry), add a drop of neutral resin, and mount with a coverslip.

Staining Results
: Elastic fibers appear dark blue to blackish-blue;
Collagen fibers appear red;
Muscle fibers and erythrocytes appear yellow.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[Weigert's Elastic Fiber Staining Kit]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.