2. Metabolic Enzyme/Protease Anti-infection TGF-beta/Smad Stem Cell/Wnt Neuronal Signaling Membrane Transporter/Ion Channel Cell Cycle/DNA Damage Immunology/Inflammation
  3. Phosphodiesterase (PDE) Bacterial PKA Tyrosinase TRP Channel Microphthalmia Associated Transcription Factor (MITF) Interleukin Related
  4. Tribuloside

Tribuloside is a flavonoid and PDE4 inhibitor (IC50: 6 μM). Tribuloside exhibits antibacterial and antioxidant activities, capable of scavenging DPPH free radicals. Tribuloside also promotes melanogenesis. Tribuloside can be used in the research of hypopigmentary diseases, sun protection, and inflammatory diseases such as acute lung injury.

For research use only. We do not sell to patients.

Tribuloside Chemical Structure

Tribuloside Chemical Structure

CAS No. : 22153-44-2

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Customer Review

Based on 1 publication(s) in Google Scholar

Tribuloside Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Tribuloside

View All Phosphodiesterase (PDE) Isoform Specific Products:

View All Isoforms
PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

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PKA PKAR

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TRPC TRPM TRPV TRPA TRPML

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IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tribuloside is a flavonoid and PDE4 inhibitor (IC50: 6 μM). Tribuloside exhibits antibacterial and antioxidant activities, capable of scavenging DPPH free radicals. Tribuloside also promotes melanogenesis. Tribuloside can be used in the research of hypopigmentary diseases, sun protection, and inflammatory diseases such as acute lung injury[1][2][3].

IC50 & Target[2][3]

PDE4

6 μM (IC50)

IL-1β

 

IL-6

 

Cellular Effect
Cell Line Type Value Description References
HepG2 EC50
0.155 μM
Compound: Tiliroside
Increase in glucose consumption in insulin-resistant human HepG2 cells after 24 hrs
Increase in glucose consumption in insulin-resistant human HepG2 cells after 24 hrs
[PMID: 21856048]
Monocyte IC50
5.2 μM
Compound: tiliroside
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
[PMID: 8882428]
In Vitro

Tribuloside exhibits antimycobacterial activity against non-pathogenic Mycobacterium madagascariense and Mycobacterium indicus pranii with MIC values of 0.8-1.6 mg/mL[1].
Tribuloside (0-20 μM; 48 h) significantly increases melanogenesis, promotes dendrite formation, and enhances melanosome maturation and transport in human epidermal melanocytes, which is associated with the PDE/cAMP/PKA pathway[2].
Tribuloside (0-20 μM; 0-48 h) upregulates the expression of proteins related to melanogenesis and melanosome transport in human epidermal melanocytes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tribuloside Related Antibodies

Western Blot Analysis[2]

Cell Line: Human epidermal melanocytes (HEMCs)
Concentration: 0, 5, 10 and 20 μM
Incubation Time: 0, 4, 12, 24 and 48 h
Result: Increased the levels of PMEL-17, tyrosinase, TRP-1, TRP-2 and MITF in a time- and dose-dependent manner.
Raised the levels of Rab27a, Rab17 and Cdc42 in a time- and dose-dependent manner.
In Vivo

Tribuloside (5-20 μM; dissolved in embryo medium; single addition; 35-60 h) reverses hypopigmentation and increases melanin content in zebrafish embryo depigmentation models induced by 1-Phenyl-2-thiourea[2].
Tribuloside (10 mg/kg; intraperitoneal injection; single dose) improves LPS (HY-D1056)-induced acute lung injury in mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male mice aged 6 weeks old (30-40 g) treated LPS (HY-D1056)[3]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; single dose
Result: Significantly alleviated lung tissue inflammation and fibrosis, repaired damaged alveolar structure, increased the number of alveoli and the percentage of alveolar area.
Reduced the expression levels of inflammatory cytokines IL-1β, TNF-α, and IL-6 in bronchoalveolar lavage fluid.
Molecular Weight

594.52

Formula

C30H26O13
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O[C@@H]2O[C@@H]([C@@H](O)[C@H](O)[C@H]2O)COC(/C=C/C3=CC=C(O)C=C3)=O)=C(C4=CC=C(O)C=C4)OC5=CC(O)=CC(O)=C51
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (210.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6820 mL 8.4101 mL 16.8203 mL
5 mM 0.3364 mL 1.6820 mL 3.3641 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.64%

SDS (251 KB)

COA (263 KB) HNMR (317 KB) LCMS (268 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6820 mL 8.4101 mL 16.8203 mL 42.0507 mL
5 mM 0.3364 mL 1.6820 mL 3.3641 mL 8.4101 mL
10 mM 0.1682 mL 0.8410 mL 1.6820 mL 4.2051 mL
15 mM 0.1121 mL 0.5607 mL 1.1214 mL 2.8034 mL
20 mM 0.0841 mL 0.4205 mL 0.8410 mL 2.1025 mL
25 mM 0.0673 mL 0.3364 mL 0.6728 mL 1.6820 mL
30 mM 0.0561 mL 0.2803 mL 0.5607 mL 1.4017 mL
40 mM 0.0421 mL 0.2103 mL 0.4205 mL 1.0513 mL
50 mM 0.0336 mL 0.1682 mL 0.3364 mL 0.8410 mL
60 mM 0.0280 mL 0.1402 mL 0.2803 mL 0.7008 mL
80 mM 0.0210 mL 0.1051 mL 0.2103 mL 0.5256 mL
100 mM 0.0168 mL 0.0841 mL 0.1682 mL 0.4205 mL
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Tribuloside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tribuloside22153-44-2Phosphodiesterase (PDE)BacterialPKATyrosinaseTRP ChannelMicrophthalmia Associated Transcription Factor (MITF)Interleukin RelatedProtein kinase ATransient receptor potential channelsMicrophthalmia Associated Transcription FactorILdepigmentation modelacute lung injury modelMycobacterium madagascarienseMycobacterium indicus praniihuman epidermal melanocytesInhibitorinhibitorinhibit

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