TRFS-red 

TRFS-red, a red fluorescence emission off-on probe, is selective for thioredoxin reductase (TrxR). TRFS-red exhibits high response rate and sensitivity. TRFS-red can be used for imaging live cells^[1].

TRFS-red (1 μM; 30-120 min) gives red fluorescence signal in live HeLa cells, and the red fluorescence is predominantly distributed in the cytosol^[1].
TRFS-red (10 μM) has a maximal absorption at ~530 nm in TE bu er (50 mM Tris-HCl and 1 mM EDTA, pH 7.4)^[1].
TRFS-red displays a reliable fluorescence signal in the pH range of 5.5-8.5^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

682.12

C₂₈H₂₈ClN₃O₁₁S₂

Solid

Brown to black

O=C(NC1=CC2=C(N=C3C(O2)=C/C(C=C3)=[N+](CCC(OC)=O)/CCC(OC)=O)C4=C1C=CC=C4)OC5CSSC5.O=Cl(=O)([O-])=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Ma H, et, al. A fast response and red emission probe for mammalian thioredoxin reductase. Chem Commun (Camb). 2016 Oct 4;52(81):12060-12063.  [Content Brief]