2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species (ROS)
  4. TBE56

TBE56, a molecular glue, is a BACH1 degrader, with an EC50 of 44 nM. TBE56 is a weak NRF2 inducer and the biotinylated TBE31. TBE56 interacts and promotes the degradation of BACH1 via a mechanism involving the E3 ligase FBX022. TBE56 reduces intracellular Fe2+ accumulation, ROS generation, and malondialdehyde (MDA) content, while increasing GSH/GSSG ratio and upregulating GPX4 in Prominin-2-overexpressed BMSCs. TBE56 significantly ameliorates intervertebral disc degeneration (IVDD) in puncture-induced SD rat IVDD model. TBE56 can be used for the study of intervertebral disc degeneration (IVDD).

For research use only. We do not sell to patients.

TBE56

TBE56 Chemical Structure

CAS No. : 1459836-79-3

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

TBE56 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

TBE56, a molecular glue, is a BACH1 degrader, with an EC50 of 44 nM. TBE56 is a weak NRF2 inducer and the biotinylated TBE31. TBE56 interacts and promotes the degradation of BACH1 via a mechanism involving the E3 ligase FBX022. TBE56 reduces intracellular Fe2+ accumulation, ROS generation, and malondialdehyde (MDA) content, while increasing GSH/GSSG ratio and upregulating GPX4 in Prominin-2-overexpressed BMSCs. TBE56 significantly ameliorates intervertebral disc degeneration (IVDD) in puncture-induced SD rat IVDD model. TBE56 can be used for the study of intervertebral disc degeneration (IVDD)[1][2].

In Vitro

TBE56 (100 nM, 3 h) significantly reduces BACH1 protein levels with an EC50 of 44 nM in HaCaT cells[1].
TBE56 (100 nM, 16 h) induces HMOX1 mRNA expression in HaCaT cells[1].
TBE56 (0.05-5 μM, 5-16 h) induces HMOX1 mRNA expression, promotes BACH1 degradation, and reduces nuclear BACH1 levels in A549, H1299, MDA-MB-231, and MDA-MB-468 cells in a concentration-dependent manner[1].
TBE56 (100 nM, 6 h) reduces migration and invasion of wild-type MDA-MB-231 cells[1].
TBE56 (110 nM, 3 h pretreatment) significantly decreases intracellular Fe2+ accumulation and ROS generation in Prominin-2-overexpressed BMSCs[2].
TBE56 (110 nM, 3 h pretreatment) significantly increases GSH/GSSG ratio and decreases MDA content in Prominin-2-overexpressed BMSCs[2].
TBE56 (110 nM, 16 h pretreatment) significantly improves viability and reduces LDH release in degenerative NPCs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TBE56 Related Antibodies

Western Blot Analysis[1]

Cell Line: HaCaT cells
Concentration: 100 nM
Incubation Time: 3 h
Result: Reduced BACH1 protein levels with an EC50 of 44 nM in HaCaT cells.
In Vivo

TBE56 (pretreatment of Prominin-2-overexpressed BMSCs, 1×104 cells/μL, 10 μL per rat, intradiscal injection, every 2 weeks for 2 months) significantly ameliorates intervertebral disc degeneration (IVDD) in puncture-induced SD rat IVDD model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Four-week-old male Sprague-Dawley (SD) rats (160–180 g): A needle was punctured into the center of lumbar IVD segments to induce disc degeneration, and SD rats’ forelimbs were amputated with the food trough raised post-surgery for 1-week recovery, establishing a puncture-induced SD rat IVDD model [1]
Dosage: Pretreatment of Prominin-2-overexpressed BMSCs, 1×104 cells/μL, 10 μL per rat,
Administration: Intradiscal injection, every 2 weeks for 2 months
Result: Achieved enhanced retention of transplanted Prominin-2-overexpressed BMSCs in degenerated IVDs of SD rats.
Showed no significant changes in body weight.
Reduced the histological scores of IVD degeneration in SD rats.
Appeared elevated disc height index (DHI) percentage.
Showed greater amelioration of IVD degeneration with downregulated protein levels of MMP-9, ADAMTS5, and MMP-13 in degenerated IVD tissues of SD rats.
Achieved improved histological morphology of IVDs.
Reduced lipid peroxidation and oxidative stress in degenerated IVD microenvironment.
Molecular Weight

685.83

Formula

C37H43N5O6S
CAS No.
SMILES

C#C[C@@]12C([C@]3([C@]([C@](C)(C(C(C#N)=C3)=O)COC(CCCCCNC(CCCC[C@H]4[C@]5([H])[C@](NC(N5)=O)([H])CS4)=O)=O)([H])CC1)C)=CC(C(C#N)=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

TBE56 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TBE561459836-79-3TBE 56TBE-56Reactive Oxygen Species (ROS)ROSGSH/GSSGintervertebral disc degenerationBMSCsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
TBE56
Cat. No.:
HY-17655
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.