Sulfo Cy7 tyramide  (Synonyms: Sulfo Cy7(Et) tyramide)

Sulfo Cy7 tyramide, a red fluorescent dye (Ex=740 nm, Em=770 nm), is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). Sulfo Cy7 tyramide can be used for multiplex Immunohistochemistry (mIHC)^[1].

Sulfo Cy7 tyramide assay
a. Cells are fixed with 3.7% paraformaldehyde in 1x PBS at RT for 20 minutes, and then rinse with 1x PBS twice.
b. permeabilize in 0.1% Triton X-100 for 1-5 minutes at RT, and then rinse with 1x PBS twice.
c. Incubate in 3% H₂O₂ in 1x PBS for 10 min to block endogenous peroxidase activity, and then rinse with 1x PBS twice.
d. Incubate in 1x PBS plus 1% BSA for 30 min at 4℃.
e. Apply the primary antibodies working solution for 1 h at RT or overnight at 4 °C, and then rinse with 1x PBS three times, 5 minutes each.
f. Apply 100 µL secondary antibody-HRP working solution for 1 h at RT and then rinse with 1x PBS three times, 5 minutes each.
g. Incubate in 100 µL Sulfo Cy7 tyramide working solution for 5-10 minutes at RT, and then rinse with 1x PBS three times, 5 minutes each.
h. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of Sulfo Cy7 tyramide working solution according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

802.01

C₄₃H₅₁N₃O₈S₂

Solid

Brown to reddish brown

O=C(CCCCC[N+]1=C(/C=C/C=C/C=C/C=C2N(CC)C3=C(C/2(C)C)C=C(S(=O)(O)=O)C=C3)C(C)(C)C4=CC(S(=O)([O-])=O)=CC=C14)NCCC5=CC=C(C=C5)O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Clutter MR, et al. Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry. Cytometry A. 2010 Nov; 77(11):1020-31.  [Content Brief]