2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation Apoptosis
  3. Aldose Reductase Heme Oxygenase (HO) Reactive Oxygen Species Apoptosis
  4. Soyasaponin Bb

Soyasaponin Bb is an orally active, covalent inducer of heme oxygenase HO-1 and an inhibitor of aldose reductase AKR1B1. Soyasaponin Bb can regulate oxidative stress pathways, enhance antioxidant capacity, reduce reactive oxygen species (ROS) generation, and inhibit lipid peroxidation and hepatocyte apoptosis. Soyasaponin Bb improves alcohol-induced hepatocyte membrane damage and liver function abnormalities, and improves Scopolamine (HY-N0296)-induced memory impairment. Soyasaponin Bb has antioxidant, hepatoprotective, and neuroprotective activities.

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Soyasaponin Bb Chemical Structure

Soyasaponin Bb Chemical Structure

CAS No. : 51330-27-9

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Based on 1 publication(s) in Google Scholar

Other Forms of Soyasaponin Bb:

Soyasaponin Bb Related Antibodies

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1 Publications Citing Use of MCE Soyasaponin Bb

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Description

Soyasaponin Bb is an orally active, covalent inducer of heme oxygenase HO-1 and an inhibitor of aldose reductase AKR1B1. Soyasaponin Bb can regulate oxidative stress pathways, enhance antioxidant capacity, reduce reactive oxygen species (ROS) generation, and inhibit lipid peroxidation and hepatocyte apoptosis. Soyasaponin Bb improves alcohol-induced hepatocyte membrane damage and liver function abnormalities, and improves Scopolamine (HY-N0296)-induced memory impairment. Soyasaponin Bb has antioxidant, hepatoprotective, and neuroprotective activities[1][2][3][4].

IC50 & Target

HO-1

 

In Vitro

Soyasaponin Bb (25-100 μg/mL; pretreatment for 24 h + ethanol treatment for 24 h) increases cell viability, reduces reactive oxygen species (ROS) levels, increases superoxide dismutase (SOD) and glutathione (GSH) activities, downregulates malondialdehyde (MDA) and alanine aminotransferase (ALT) levels, and induces heme oxygenase-1 (HO-1) expression in rat hepatocyte BRL 3A assay[1].
Soyasaponin Bb (2.36-94.30 mg/L; 4 h) shows no significant cytotoxicity in the Caco-2 intestinal epithelial cell experiment. Soyasaponin Bb has an apparent permeability (Papp) across the membrane of less than 1.0×10-6 cm/s), with the apical-to-basolateral (AP-BL) transport significantly higher than the basolateral-to-apical (BL-AP) transport, indicating that the cell permeation of Soyasaponin Bb is mainly passive transport[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Soyasaponin Bb Related Antibodies

Cell Viability Assay[1]

Cell Line: Rat BRL 3A hepatocytes
Concentration: 0, 25, 50, 100 μg/mL
Incubation Time: 24 h pretreatment with Soyasaponin Bb, followed by 24 h ethanol (250 mmol/L) treatment
Result: Dose-dependently increased cell viability, with 50 and 100 μg/mL groups showing significant protection compared to the ethanol-alone group.
The 25 μg/mL group showed minimal improvement.
In Vivo

Soyasaponin Bb (100 mg/kg; oral administration; single dose) showes a peak plasma drug concentration of 19.8 ng/mL in the Sprague-Dawley rat model. It was mainly excreted in the feces, with very little excretion in the urine. The metabolite Soyasapogenol B was only detected in the feces[2].
Soyasaponin Bb (10 mg/kg; oral administration; once a day; for 5 consecutive days) significantly improves passive avoidance, Y-maze and Morris water maze task performance in the Scopolamine (HY-N0296)-induced mouse memory impairment model, increases brain-derived neurotrophic factor (BDNF) expression and cyclic adenosine monophosphate response element binding protein (CREB) phosphorylation level, without inhibiting acetylcholinesterase (AChE) activity[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Scopolamine-induced ICR mice Memory Impairment Model (male; 18-22 g, 5 weeks old)[3]
Dosage: 10 mg/kg
Administration: Oral gavage, once 1 hour before scopolamine injection, single dose
Result: Significantly increased the latency time to enter the dark compartment in the retention trial, restoring memory impairment to 97% of the untreated normal control level, comparable to the positive control tacrine (10 mg/kg).
No significant differences were observed during the acquisition trial.
Molecular Weight

943.12

Formula

C48H78O18
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H]([C@H]1O[C@@]2([H])[C@H](O[C@]3([H])O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@@H](O)[C@@H](O)[C@@H](CO)O2)[C@H](O)[C@@H](C(O)=O)O[C@@]1([H])O[C@@H]4[C@@](CO)(C)[C@]5([H])CC[C@@]6(C)[C@]7(C)CC[C@@]8(C)[C@H](O)CC(C)(C)C[C@@]8([H])C7=CC[C@]6([H])[C@@]5(C)CC4
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (106.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0603 mL 5.3016 mL 10.6031 mL
5 mM 0.2121 mL 1.0603 mL 2.1206 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.13%

SDS (252 KB)

COA (258 KB) RP-HPLC (287 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.0603 mL 5.3016 mL 10.6031 mL 26.5078 mL
5 mM 0.2121 mL 1.0603 mL 2.1206 mL 5.3016 mL
10 mM 0.1060 mL 0.5302 mL 1.0603 mL 2.6508 mL
15 mM 0.0707 mL 0.3534 mL 0.7069 mL 1.7672 mL
20 mM 0.0530 mL 0.2651 mL 0.5302 mL 1.3254 mL
25 mM 0.0424 mL 0.2121 mL 0.4241 mL 1.0603 mL
30 mM 0.0353 mL 0.1767 mL 0.3534 mL 0.8836 mL
40 mM 0.0265 mL 0.1325 mL 0.2651 mL 0.6627 mL
50 mM 0.0212 mL 0.1060 mL 0.2121 mL 0.5302 mL
60 mM 0.0177 mL 0.0884 mL 0.1767 mL 0.4418 mL
80 mM 0.0133 mL 0.0663 mL 0.1325 mL 0.3313 mL
100 mM 0.0106 mL 0.0530 mL 0.1060 mL 0.2651 mL
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Soyasaponin Bb Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Soyasaponin Bb51330-27-9Aldose ReductaseHeme Oxygenase (HO)Reactive Oxygen SpeciesApoptosisHeme OxygenaseInhibitorinhibitorinhibit

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