2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. Mitochondrial Metabolism Oxidative Phosphorylation Reactive Oxygen Species MMP
  4. SCAL-255

SCAL-255 is a potent mitochondrial complex I (CI) inhibitor with an IC50 of 1.14 μM. SCAL-255 blocks mitochondrial function, inhibits oxygen consumption rate (OCR), induces reactive oxygen species (ROS) production, and reduces MMP. SCAL-255 can be used in the research of oxidative phosphorylation (OXPHOS)-dependent cancers, such as colorectal cancer (CRC) and acute myeloid leukemia (AML), etc.

For research use only. We do not sell to patients.

SCAL-255 Chemical Structure

SCAL-255 Chemical Structure

CAS No. : 2798953-61-2

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

SCAL-255 Related Antibodies

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View All MMP Isoform Specific Products:

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MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

SCAL-255 is a potent mitochondrial complex I (CI) inhibitor with an IC50 of 1.14 μM. SCAL-255 blocks mitochondrial function, inhibits oxygen consumption rate (OCR), induces reactive oxygen species (ROS) production, and reduces MMP. SCAL-255 can be used in the research of oxidative phosphorylation (OXPHOS)-dependent cancers, such as colorectal cancer (CRC) and acute myeloid leukemia (AML), etc[1].

IC50 & Target

IC50: 1.14 μM (Mitochondrial complex I)[1]
In Vitro

SCAL-255 (0.01-10 nM; 96 h) significantly inhibits the proliferation of human colorectal cancer cell line HCT116 and acute myeloid leukemia cell line KG-1. SCAL-255 has little inhibitory effect on NCM460 cells[1].
SCAL-255 (2 nM, 10 nM; 24 h) inhibits the oxygen consumption rate (OCR), activates glycolysis, and changes the energy metabolism of HCT116 cells[1].
SCAL-255 (0.13-2 nM) induces the production of ROS and reduces the MMP in HCT116 and KG-1 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SCAL-255 Related Antibodies

Cell Viability Assay[1]

Cell Line: HCT116, KG-1 cells
Concentration: 0.01 nM, 0.1 nM, 1 nM, 10 nM
Incubation Time: 96 h
Result: Ssignificantly inhibited the proliferation of HCT116 and KG-1 cells.
In Vivo

SCAL-255 (5 mg/kg; intraperitoneal injection; five consecutive days per week for 24 days) inhibits tumor growth in the KG-1 subcutaneous xenograft tumor model of NOD-SCID mice, with a tumor growth inhibition rate of 61.5%, and has no significant effects on the body weight, blood biochemical parameters, routine blood indices, and histology of major organs of the mice[1].
The maximum tolerated dose (MTD) values of SCAL-255 is 68 mg/kg in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female NOD-SCID mice (18-20 g, 5 weeks) inoculated with KG-1 cells[1].
Dosage: 5 mg/kg
Administration: Intraperitoneal injection, five consecutive days per week for 24 days
Result: Exhibited adequate antitumor activity with a tumor growth inhibitory (TGI) rate of 61.5%.
No significant change of body weight, blood biochemical parameters, routine blood indexes, and histology of major organs were observed.
Molecular Weight

527.54

Formula

C27H28F3N5O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=NN(CC2=CC=CC(N3CCN(C(C4CC4)=O)CC3)=C2)C(C)=C1)NC5=CC=C(OC(F)(F)F)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (94.78 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8956 mL 9.4780 mL 18.9559 mL
5 mM 0.3791 mL 1.8956 mL 3.7912 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8956 mL 9.4780 mL 18.9559 mL 47.3898 mL
5 mM 0.3791 mL 1.8956 mL 3.7912 mL 9.4780 mL
10 mM 0.1896 mL 0.9478 mL 1.8956 mL 4.7390 mL
15 mM 0.1264 mL 0.6319 mL 1.2637 mL 3.1593 mL
20 mM 0.0948 mL 0.4739 mL 0.9478 mL 2.3695 mL
25 mM 0.0758 mL 0.3791 mL 0.7582 mL 1.8956 mL
30 mM 0.0632 mL 0.3159 mL 0.6319 mL 1.5797 mL
40 mM 0.0474 mL 0.2369 mL 0.4739 mL 1.1847 mL
50 mM 0.0379 mL 0.1896 mL 0.3791 mL 0.9478 mL
60 mM 0.0316 mL 0.1580 mL 0.3159 mL 0.7898 mL
80 mM 0.0237 mL 0.1185 mL 0.2369 mL 0.5924 mL
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SCAL-255 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SCAL-2552798953-61-2SCAL255SCAL 255Mitochondrial MetabolismOxidative PhosphorylationReactive Oxygen SpeciesMMPMatrix metalloproteinasesmitochondrial complex ImitochondrialOCRROSMMP. SCAL-266antiproliferationoxidative phosphorylationOXPHOSInhibitorinhibitorinhibit

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