2. Epigenetics TGF-beta/Smad MAPK/ERK Pathway
  3. PKC JNK
  4. Ro 31-8220 formic

Ro 31-8220 formic  (Synonyms: Bisindolylmaleimide IX formic)

Cat. No.: HY-13866B Purity: 95.98%
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Ro 31-8220 formic is a potent PKC inhibitor, with IC50s of 5, 24, 14, 27, 24 and 23 nM for PKCα, PKCβI, PKCβII, PKCγ, PKCε and rat brain PKC, respectively. Ro 31-8220 formic also significantly inhibits MAPKAP-K1b, MSK1, S6K1 and GSK3β (IC50s, 3, 8, 15, and 38 nM, respectively). Ro 31-8220 formic can also inhibit the expression of MKP-1, induce the expression of c-Jun, and activate JNK, and these effects possess pharmacological properties independent of PKC.

For research use only. We do not sell to patients.

Ro 31-8220 formic

Ro 31-8220 formic Chemical Structure

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Based on 15 publication(s) in Google Scholar

Other Forms of Ro 31-8220 formic:

Ro 31-8220 formic Related Antibodies

Top Publications Citing Use of Products

    Ro 31-8220 formic purchased from MedChemExpress. Usage Cited in: Nat Commun. 2018 Sep 11;9(1):3688.  [Abstract]

    Levels of phosphorylated FAK and AKT are reduced by the PKN1 inhibitors. Cells are seeded in six-well plates after transfection with PKN1 inhibitors Lestaurtinib and Ro318220 or DMSO as control.

    Ro 31-8220 formic purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2017 Nov;16(5):5924-5930.  [Abstract]

    The PKC inhibitor Ro 31 8220 is used to investigate the implication of PKC mediated signaling pathways.

    View All PKC Isoform Specific Products:

    View All Isoforms
    PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

    View All JNK Isoform Specific Products:

    View All Isoforms
    JNK1 JNK2 JNK3 JNK

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ro 31-8220 formic is a potent PKC inhibitor, with IC50s of 5, 24, 14, 27, 24 and 23 nM for PKCα, PKCβI, PKCβII, PKCγ, PKCε and rat brain PKC, respectively. Ro 31-8220 formic also significantly inhibits MAPKAP-K1b, MSK1, S6K1 and GSK3β (IC50s, 3, 8, 15, and 38 nM, respectively). Ro 31-8220 formic can also inhibit the expression of MKP-1, induce the expression of c-Jun, and activate JNK, and these effects possess pharmacological properties independent of PKC[1][2][3][4][5].

    IC50 & Target

    PKC-α

    5 nM (IC50)

    PKC-βII

    14 nM (IC50)

    PKC-βI

    24 nM (IC50)

    PKC-ε

    24 nM (IC50)

    PKC-γ

    27 nM (IC50)

    Rat Brain PKC

    23 nM (IC50)

    Molecular Weight

    503.57

    Formula

    C26H25N5O4S
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    NC(SCCCN1C=C(C2=C(C3=CN(C)C4=C3C=CC=C4)C(NC2=O)=O)C5=C1C=CC=C5)=N.OC=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (198.58 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9858 mL 9.9291 mL 19.8582 mL
    5 mM 0.3972 mL 1.9858 mL 3.9716 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9858 mL 9.9291 mL 19.8582 mL 49.6455 mL
    5 mM 0.3972 mL 1.9858 mL 3.9716 mL 9.9291 mL
    10 mM 0.1986 mL 0.9929 mL 1.9858 mL 4.9646 mL
    15 mM 0.1324 mL 0.6619 mL 1.3239 mL 3.3097 mL
    20 mM 0.0993 mL 0.4965 mL 0.9929 mL 2.4823 mL
    25 mM 0.0794 mL 0.3972 mL 0.7943 mL 1.9858 mL
    30 mM 0.0662 mL 0.3310 mL 0.6619 mL 1.6549 mL
    40 mM 0.0496 mL 0.2482 mL 0.4965 mL 1.2411 mL
    50 mM 0.0397 mL 0.1986 mL 0.3972 mL 0.9929 mL
    60 mM 0.0331 mL 0.1655 mL 0.3310 mL 0.8274 mL
    80 mM 0.0248 mL 0.1241 mL 0.2482 mL 0.6206 mL
    100 mM 0.0199 mL 0.0993 mL 0.1986 mL 0.4965 mL
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    Ro 31-8220 formic Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ro 31-8220Bisindolylmaleimide IXPKCJNKProtein kinase Cc-Jun N-terminal kinaseInhibitorinhibitorinhibit

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