1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Topoisomerase
  4. DNA topoisomerase II
  5. TOP2A Protein, Human

TOP2A Protein, Human is the recombinant human-derived TOP2A protein, expressed by E. coli, with tag free tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Stock
50 μg   Get quote  
100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

TOP2A Protein, Human is the recombinant human-derived TOP2A protein, expressed by E. coli, with tag free tag.

Background

TOP2A is a key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules. It generates a double-stranded break in one of the strands, passes the intact strand through the broken strand, and religates the broken strand. Additionally, TOP2A may play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).

Species

Human

Source

E. coli

Tag

Tag Free

Accession

P11388-1 (S1354-D1473)

Gene ID

7153

Molecular Construction
N-term
TOP2A (S1354-S1473)
Accession # P11388-1
C-term
Protein Length

Partial

Synonyms
TOP2A; DNA topoisomerase 2-alpha; TOP2; DNA topoisomerase II; alpha isozyme
AA Sequence

SPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDD

Predicted Molecular Mass
12.8 kDa
Molecular Weight

Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

Structure/Form
Monomer
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

TOP2A Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
TOP2A Protein, Human
Cat. No.:
HY-P704742
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