TMEM175 Protein, Human (HEK293)

TMEM175 Protein, Human (HEK293) is the recombinant human-derived TMEM175, expressed by HEK293 , with tag Free labeled tag. ,

TMEM175 is a proton-activated proton channel that facilitates proton efflux from endosomes and lysosomes to maintain steady-state pH. It is activated at low pH (below 4.6) and selectively mediates lysosomal proton release, balancing V-ATPase activity for pH homeostasis (by similar: PubMed:35750034). Additionally, TMEM175 functions as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes. It serves as the pore-forming subunit of the lysoK(GF) complex, which consists of TMEM175 and AKT (AKT1, AKT2, or AKT3) and is activated by extracellular growth factors. The lysoK(GF) complex opens the TMEM175 channel via AKT-induced conformational changes, activating its function and playing a critical role in protecting neurons from stress-induced damage (by similar: PubMed:33505021).

Human

HEK293

Tag Free

Q9BSA9-1 (M1-C504)

84286

Full Length of Isoform-1

Approximately 56.3 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 8.0, 150 mM KCl, 2 mM DTT, 0.1% (w/v) LMNG.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.