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  4. SMO Protein, Human (sf9, Flag, His)

SMO Protein, Human (sf9, Flag, His) is the recombinant human-derived SMO, expressed by sf9 insect cells , with His, Flag labeled tag. ,

For research use only. We do not sell to patients.

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Description

SMO Protein, Human (sf9, Flag, His) is the recombinant human-derived SMO, expressed by sf9 insect cells , with His, Flag labeled tag. ,
Background

SMO is a G protein-coupled receptor that associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors, which releases the GLI proteins and allows transcriptional activation of hedgehog pathway target genes (By similarity). SMO is also required for the accumulation of KIF7, GLI2, and GLI3 in the cilia (PubMed:19592253) and interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (By similarity).
Species

Human
Source

Sf9 insect cells
Tag

His;Flag
Accession

Q99835 (S190-Q555)
Gene ID

6608
Protein Length

Partial
Synonyms
SMOH
Predicted Molecular Mass
56.1 kDa
Purity

Greater than 80% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SMO Protein, Human (sf9, Flag, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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