S100A1 Protein, Mouse (His)

S100A1 is a small calcium-binding protein that plays an important regulatory role in Ca(2+) homeostasis, chondrocyte biology, and cardiomyocyte function.In response to elevated intracellular Ca(2+) levels, S100A1 undergoes conformational changes and interacts with key proteins in cardiomyocytes, such as RYR1, RYR2, ATP2A2, and F1-ATPase.S100A1 Protein, Mouse (His) is the recombinant mouse-derived S100A1 protein, expressed by E.coli , with N-His labeled tag.

S100A1 is a small calcium-binding protein that plays pivotal roles in various biological processes, including the regulation of Ca(2+) homeostasis, chondrocyte biology, and cardiomyocyte function. In response to elevated intracellular Ca(2+) levels, S100A1 undergoes conformational changes, enabling interactions with specific target proteins and modulating their activities. In cardiomyocytes, it orchestrates a network that governs sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function by interacting with key proteins such as ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2, and mitochondrial F1-ATPase. This multifaceted protein contributes to diastolic Ca(2+) dissociation and myofilament mechanics, enhancing relaxation during diastole. S100A1 forms dimers of either two alpha chains, two beta chains, or one of each, and it also engages in heterodimeric interactions with S100P. Additionally, it interacts with various proteins such as AGER, CAPZA1, FKBP4, RYR1, RYR2, CACYBP, PPP5C, ATP2A2, PLN, ATP5F1A, and ATP5F1B, participating in diverse cellular processes in a calcium-dependent manner.

Mouse

E. coli

N-His

P56565 (G2-S94)

20193  [NCBI]

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.