ROBO1 Protein, Human (HEK293, His)

The ROBO1 protein is a receptor for SLIT1 and SLIT2 and plays a key role in cellular responses during migration, including axonal navigation and projection. Interaction with FLRT3 promotes axonal attraction to NTN1-expressing cells. ROBO1 Protein, Human (HEK293, His) is the recombinant human-derived ROBO1 protein, expressed by HEK293, with C-His labeled tag.

The ROBO1 Protein serves as a receptor for SLIT1 and SLIT2, playing a pivotal role in mediating cellular responses to molecular guidance cues during cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons during neuronal development. Its interaction with the intracellular domain of FLRT3 facilitates axon attraction towards cells expressing NTN1, and in axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may involve the formation of a ROBO1-DCC complex. Additionally, ROBO1 Protein contributes to the regulation of cell migration through its interaction with MYO9B, inhibiting MYO9B-mediated stimulation of RHOA GTPase activity and consequently leading to increased levels of active, GTP-bound RHOA. This receptor may also play a role in lung development. Existing as a homodimer, dimerization is facilitated by the extracellular domain and is independent of SLIT liganding. ROBO1 Protein interacts with SLIT1, SLIT2, FLRT3, and MYO9B, indicating its diverse involvement in cellular signaling pathways.

Human

HEK293

C-His

Q9Y6N7-1 (Q26-P897)

6091

Extracellular Domain

QLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQP

Approximately 100-120 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.