PRKCD Protein, Human (sf9, GST)

PRKCD Protein, Human (sf9, GST) is the recombinant human-derived PRKCD, expressed by Sf9 insect cells , with N-GST labeled tag. ,

PKC delta, a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase, plays dual roles in cell death and survival, acting as a pro-apoptotic protein during DNA damage-induced apoptosis and an anti-apoptotic protein during cytokine receptor-initiated cell death. It is implicated in tumor suppression, required for oxygen radical production by NADPH oxidase, and functions as a positive or negative regulator in platelet responses. PKC delta negatively regulates B cell proliferation and plays a crucial role in self-antigen-induced B cell tolerance induction. Under various cellular stresses, PKC delta activates signaling pathways leading to apoptosis, including the activation of death-promoting transcription factor BCLAF1/Btf, modulation of the CHUK/IKKA-p53 axis, and formation of a complex with ABL1. Moreover, it regulates cell cycle progression, mediates the inhibition of cell cycle progression at G1/S phase, and activates the G2/M DNA damage checkpoint. PKC delta is also involved in the control of cell cycle progression and protection of glioma cells from TNFSF10/TRAIL-induced apoptosis. Furthermore, it participates in antifungal immunity and regulates NADPH oxidase activity in neutrophils and eosinophils. PKC delta plays a critical role in platelet functions, acting as a negative regulator of filopodia formation and modulating granule secretion downstream of specific receptors. Its extensive phosphorylation targets, such as MUC1, ELAVL1, mitochondrial phospholipid scramblase 3 (PLSCR3), and SMPD1, underscore its versatility in modulating diverse cellular processes.

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of PKCδ was 154.7 µM.

Human

Sf9 insect cells

N-GST

Q05655-1 (A2-D676)

5580

Partial

APFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

Approximately 104.0 KDa.

Greater than 80 % as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.