PDIA3 Protein, Human (HEK293, His)

The PDIA3 protein is a disulfide isomerase that catalyzes disulfide bonding processes and serves as a key protein folding partner. It is the core of the MHC I peptide loading complex and is essential for TAPBP folding. PDIA3 Protein, Human (HEK293, His) is the recombinant human-derived PDIA3, expressed by HEK293, with C-6*His labeled tag.

PDIA3, a protein disulfide isomerase, plays a pivotal role in the intricate processes of catalyzing disulfide bond formation, isomerization, and reduction or oxidation in client proteins, functioning concurrently as a crucial protein folding chaperone. Within the major histocompatibility complex class I (MHC I) peptide loading complex, PDIA3 assumes a central position, serving as an indispensable folding chaperone for TAPBP. Collaboratively with TAPBP, PDIA3 facilitates the dynamic assembly of the MHC I complex, ensuring the seamless integration of high-affinity antigens within the endoplasmic reticulum. Consequently, PDIA3 emerges as a key player in orchestrating the presentation of antigens to cytotoxic T cells, thereby playing a critical role in adaptive immunity.

Human

HEK293

C-6*His

P30101 (S25-L505)

2923  [NCBI]

SDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL

Approximately 58 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.